{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T09:43:25Z","timestamp":1696239805247},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,4,9]],"date-time":"2015-04-09T00:00:00Z","timestamp":1428537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2015,11]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>To study the relationship between loss of heterozygosity (LOH<\/jats:styled-content>) at the human leukocyte antigen (HLA<\/jats:styled-content>) locus and the pathogenicity and clinicopathological features of thymic epithelial tumors (TET<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Tumor and adjacent normal tissues were isolated from 36 TET<\/jats:styled-content> patients. Five microsatellite loci (D6S1666<\/jats:styled-content>, D6S265<\/jats:styled-content>, D6S273<\/jats:styled-content>, DS6276<\/jats:styled-content>, and D6S291<\/jats:styled-content>) within the HLA<\/jats:styled-content> locus were amplified by polymerase chain reaction. DNA<\/jats:styled-content> sequencing was used to measure the frequency of microsatellite LOH<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>LOH<\/jats:styled-content> was identified in at least one locus in 83.6% of TET<\/jats:styled-content> patients. LOH<\/jats:styled-content> frequency at D6S1666<\/jats:styled-content>, D6S265<\/jats:styled-content>, D6S273<\/jats:styled-content>, D6S276<\/jats:styled-content>, and D6S291<\/jats:styled-content> was 44.4%, 16.7%, 30.5%, 38.9%, and 36.1% respectively. There was no significant association between LOH<\/jats:styled-content> frequency in TET<\/jats:styled-content> with tumor severity, or in the presence or absence of myasthenia gravis.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>D6S1666<\/jats:styled-content>, D6S265<\/jats:styled-content>, D6S273<\/jats:styled-content>, DS6S276<\/jats:styled-content>, and D6S29<\/jats:styled-content> are sensitive loci for studying microsatellite LOH<\/jats:styled-content> in TET<\/jats:styled-content>. LOH<\/jats:styled-content> within the HLA<\/jats:styled-content> complex is implicated in the occurrence and development of TET<\/jats:styled-content>, with the HLA\u2010DQA1<\/jats:styled-content> gene likely involved. However, an understanding of the relationship between LOH<\/jats:styled-content> and the clinicopathological features of TET<\/jats:styled-content> requires a larger sample size than that of the present study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.12252","type":"journal-article","created":{"date-parts":[[2015,4,9]],"date-time":"2015-04-09T08:02:59Z","timestamp":1428566579000},"page":"749-753","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Loss of heterozygosity at the human leukocyte antigen locus in thymic epithelial tumors"],"prefix":"10.1111","volume":"6","author":[{"given":"Yuan","family":"Chen","sequence":"first","affiliation":[{"name":"Deparment of Cardiothoracic Surgery Tianjin Medical University General Hospital Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guojin","family":"Wang","sequence":"additional","affiliation":[{"name":"Deparment of Hematology and 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