{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:32:08Z","timestamp":1778589128622,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,1,31]],"date-time":"2016-01-31T00:00:00Z","timestamp":1454198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Malignant pleural mesothelioma (<jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content>) is an aggressive cancer refractory to current therapies. Reduced expression of micro ribonucleic acid (<jats:styled-content style=\"fixed-case\">miR)<\/jats:styled-content>\u2010591 in a range of cancer types has suggested it is a potent tumor suppressor, and overexpression has been shown to inhibit tumor cell growth. The role of <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010591 in <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> is largely unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010591 was over\u2010expressed in vitro using micro <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> mimics in three <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> cell lines (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>513, <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>2052, <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>2373), and effects on tumor cell growth, proliferation, invasion, and target gene expression were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010591 mimic was introduced into <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> cell lines to overexpress this micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. The cellular growth, proliferation, and invasive capability was significantly inhibited after overexpression of <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010591. Growth inhibition caused by <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010591 correlated with upregulation of p21 and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>ax. Reduced invasive capability correlated with downregulation of matrix metalloproteinase\u20102 and transforming growth factor\u2010\u03b21.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010591 is a potent tumor suppressor in <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content>. Overexpression of <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010591 may represent a novel therapeutic approach for <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.12336","type":"journal-article","created":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T04:00:54Z","timestamp":1454299254000},"page":"340-347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Overexpression of micro ribonucleic acid\u2010591 inhibits cell proliferation and invasion of malignant pleural mesothelioma cells"],"prefix":"10.1111","volume":"7","author":[{"given":"Shizhao","family":"Cheng","sequence":"first","affiliation":[{"name":"Tianjin Medical University  Tianjin China"},{"name":"Department of Thoracic Surgery Tianjin Chest Hospital  Tianjin 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