{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:23:33Z","timestamp":1788344613224,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007210","name":"RWTH Aachen University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007210","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The etiology of thymic epithelial tumors is unknown. Murine polyomavirus strain PTA has been shown to induce thymomas in mice. Recently, using diverse molecular techniques, we reported the presence of human polyomavirus 7 (HPyV7) in thymic epithelial tumors. In the present study, we investigated the prevalence of Merkel cell polyomavirus (MCPyV) in thymic epithelial tumors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010six thymomas were screened for MCPyV by PCR and subsequently tested by DNA and RNA in situ hybridization and immunohistochemistry. Twenty\u2010six thymomas were diagnosed with myasthenia gravis (MG).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MCPyV DNA was detected by PCR in 7 (19.4%) of the 36 thymic epithelial tumors and in six of these, the presence of MCPyV was confirmed by fluorescence situ hybridization. Of these, 3 (28.6%) revealed weak MCPyV LT\u2010antigen protein expression. In addition, one of the MCPyV positive thymomas tested positive for MCPyV LT RNA with RNAscope. Of interest, two out of the three thymomas that previously tested positive for MCPyV by immunohistochemistry also tested positive for HPyV7. One of the 11 MG\u2010negative and 2 of the 25 MG\u2010positive were positive for MCPyV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MCPyV DNA and MCPyV protein expression can be detected in human epithelial thymoma; however, to a far lesser extent than HPyV7. Our data strongly indicate that because of its infrequent detection and weak expression, MCPyV is unlikely to play an important role in the etiopathogenesis of human thymomas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.12953","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T06:09:10Z","timestamp":1547100550000},"page":"445-451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Low prevalence of Merkel cell polyomavirus in human epithelial thymic tumors"],"prefix":"10.1111","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9231-9291","authenticated-orcid":false,"given":"Emil","family":"Chteinberg","sequence":"first","affiliation":[{"name":"Department of Pathology, GROW\u2010School for Oncology &amp; Developmental Biology Maastricht University Medical Centre+  Maastricht The Netherlands"},{"name":"Institute for Biomedical Engineering, Department of Cell Biology RWTH Aachen University Hospital  Aachen Germany"},{"name":"Department of Cell Biology, Helmholtz Institute for Biomedical Engineering RWTH Aachen University  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal","family":"Klufah","sequence":"additional","affiliation":[{"name":"Department of Pathology, GROW\u2010School for Oncology &amp; Developmental Biology Maastricht University Medical Centre+  Maastricht The Netherlands"},{"name":"Department of Laboratory Medicine, Faculty of Applied Medical Sciences Albaha University  Al\u2010Baha Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorit","family":"Rennspiess","sequence":"additional","affiliation":[{"name":"Department of Pathology, GROW\u2010School for Oncology &amp; Developmental Biology Maastricht University Medical Centre+  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mick F.","family":"Mannheims","sequence":"additional","affiliation":[{"name":"Department of Pathology, GROW\u2010School for Oncology &amp; Developmental Biology Maastricht University Medical Centre+  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myrurgia A.","family":"Abdul\u2010Hamid","sequence":"additional","affiliation":[{"name":"Department of Pathology, GROW\u2010School for Oncology &amp; Developmental Biology Maastricht University Medical Centre+  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9761-0002","authenticated-orcid":false,"given":"Mario","family":"Losen","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience Maastricht University  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlies","family":"Keijzers","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery Maastricht University Medical Centre+  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc H.","family":"De Baets","sequence":"additional","affiliation":[{"name":"Department of Neuro\u2010Science Maastricht University, School of Mental Health and Neuroscience (MHeNS)  Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Kordelia","family":"Kurz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University Hospital Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4781-0372","authenticated-orcid":false,"given":"Axel","family":"zur Hausen","sequence":"additional","affiliation":[{"name":"Department of Pathology, 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