{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:22:51Z","timestamp":1784172171528,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T00:00:00Z","timestamp":1570665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2017ZX09304026"],"award-info":[{"award-number":["2017ZX09304026"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Epidermal growth factor receptor (<jats:italic>EGFR<\/jats:italic>) gene mutation status is essential to the optimal management of lung adenocarcinoma. Liquid biopsy has advantages such as noninvasiveness, speediness, and convenience. This study aimed to detect <jats:italic>EGFR<\/jats:italic> gene mutations using next\u2010generation sequencing (NGS) from different types of body fluids from patients with lung adenocarcinoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a prospective study of 20 patients with lung adenocarcinoma recruited between January 2017 and December 2018 at the Beijing Hospital. All patients had adenocarcinoma with confirmed sensitizing <jats:italic>EGFR<\/jats:italic> mutations. Body fluid specimens included pleural effusion, ascites, pericardial effusion, and cerebrospinal fluid. NGS was conducted to test for nine lung cancer\u2010related gene in body fluid supernatant free DNA, sedimentary tumor cells, and plasma free DNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:italic>EGFR<\/jats:italic> gene mutation abundance of body fluid supernatant free DNA was higher than that of body fluid sedimentary tumor cells and plasma free DNA specimens (100% vs. 90% vs. 80%, respectively, all <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The results of <jats:italic>EGFR<\/jats:italic> mutation from the body fluid supernatants were consistent with the results from the tissue biopsy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study showed that compared with body fluid sediment tumor cells and plasma free DNA samples, body fluid supernatant free DNA has a higher detection rate and sensitivity of tumor\u2010specific mutations. Free DNA obtained from body fluid supernatants could be used as high\u2010quality specimens for gene mutation detection in patients with lung cancer. This could be applied in treatment decisions and patient management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.13201","type":"journal-article","created":{"date-parts":[[2019,10,11]],"date-time":"2019-10-11T04:09:55Z","timestamp":1570766995000},"page":"2218-2224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Detection of <i>EGFR<\/i> gene mutation status from pleural effusions and other body fluid specimens in patients with lung adenocarcinoma"],"prefix":"10.1111","volume":"10","author":[{"given":"Ping","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Oncology Beijing Hospital, National Center of Gerontology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaonan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Oncology Beijing Hospital, National Center of Gerontology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Oncology Beijing Hospital, National Center of Gerontology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Oncology Beijing Hospital, National Center of Gerontology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2934-650X","authenticated-orcid":false,"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oncology Beijing Hospital, National Center of Gerontology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdw326"},{"key":"e_1_2_7_4_1","volume-title":"NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines). 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