{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:42:34Z","timestamp":1787013754532,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T00:00:00Z","timestamp":1574640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Previous investigations have revealed that miR\u2010563 is associated with a number of diseases including the ossification of posterior longitudinal ligament, Parkinson's disease or drug resistance to leukemia. Yet, the role of miR\u2010563 and its molecular mechanism in the initiation and progression of cancers has not been previously explored. In this study, we aimed to provide clues to the function of miR\u2010563 and its direct target in lung cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Online informatics software was applied to predict the target genes of miR\u2010563. MiR\u2010563 targeting LIN28B was evaluated through the luciferase reporter gene analysis. The effect of miR\u2010563 on LIN28B at the level of RNA and protein was detected using RT\u2010PCR and immunoblotting. The ability of proliferation of human lung cancer A549 was examined by MTT assay. RNA interference targeting LIN28B was examined through immunoblotting. The level of miR\u2010563 and LIN28B and their correlation were analyzed in 27 cases of lung tumor tissues by real\u2010time PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Oncogenic LIN28B was identified as one of the target genes of miR\u2010563 in lung cancer cells. MiR\u2010563 dose\u2010dependently decreased the LIN28B RNA level and subsequently its protein level in the cells. Cell proliferation was suppressed by ectopic miR\u2010563 expression and was accelerated after endogenous miR\u2010563 was knocked down by its inhibitor. However, silence in LIN28B reversed promotion of cell proliferation by the inhibition of miR\u2010563. In lung cancer tissues, miR\u2010563 was decreased and negative correlation of miR\u2010563 and LIN28B was shown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MiR\u2010563 plays a tumor suppressive role in lung cancer progression via targeting oncogenic LIN28B.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1759-7714.13257","type":"journal-article","created":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T18:55:15Z","timestamp":1574708115000},"page":"55-61","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["MiR\u2010563 restrains cell proliferation via targeting LIN28B in human lung cancer"],"prefix":"10.1111","volume":"11","author":[{"given":"Xuefei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery The Second Hospital of Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The Second Hospital of Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The Second Hospital of Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The Second Hospital of Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desheng","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The Second Hospital of Dalian Medical University  Dalian 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