{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T04:10:51Z","timestamp":1774498251028,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T00:00:00Z","timestamp":1580860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No 81572288"],"award-info":[{"award-number":["No 81572288"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Nm23\u2010H1 gene has been found to be an inhibitor of tumor metastasis in lung cancer. MicroRNAs (miRNAs) play key roles in tumor metastasis through multiple signaling pathways. This study explored whether the nm23\u2010H1 gene could inhibit invasion and metastasis of lung cancer cells by regulating miRNA\u2010660\u20105p targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative real\u2010time PCR (qRT\u2010PCR) and western blots were used to measure the expression of nm23\u2010H1 and miR\u2010660\u20105p of various human lung cancer cell lines. Cell counting kit\u20108 (CCK\u20108), wound\u2010healing and transwell assay were carried out to assess cell proliferation, migration and invasion of each cell line. Xenograft were applied to determine in vivo effects of miR\u2010660\u20105p among nude mice. Luciferase assay and western blot were performed to determine the target gene of miR\u2010660\u20105p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that high expression of nm23\u2010H1 correlated with decreased miRNA\u2010660\u20105p expression. Inhibiting miR\u2010660\u20105p suppressed lung cancer cells progression significantly in vitro, whereas overexpression of miR\u2010660\u20105p facilitated tumor growth and bone metastasis in vivo. In addition, as the potential target gene of miR\u2010660\u20105p, SMARCA5 overexpression in vitro suppressed tumor progression and osteolytic metastasis associated RANKL signaling, which is congruent with the effect of nm23\u2010H1 on the lung cancer cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Nm23\u2010H1 inhibits tumor progression and bone\u2010specific metastasis of lung cancer by regulating miR\u2010660\u20105p\/SMARCA5\/RANKL axis, which indicates the related genes may serve as potential targets for the treatment of human lung cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p>Significant findings of the study<\/jats:p><jats:p>High expression of nm23\u2010H1 correlated with decreased miRNA\u2010660\u20105p expression. Further, downregulation of miR\u2010660\u20105p significantly suppressed the tumor progression and bone\u2010specific metastasis of lung cancer cells.<\/jats:p><jats:p>What this study adds<\/jats:p><jats:p>This is the first study to show an inverse association between nm23\u2010H1 and miR\u2010660\u20105p, and confirm that nm23\u2010H1 inhibits tumor progression and bone\u2010specific metastasis of lung cancer by regulating miR\u2010660\u20105p\/SMARCA5\/RANKL axis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.13308","type":"journal-article","created":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T10:15:50Z","timestamp":1580897750000},"page":"640-650","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Nm23\u2010H1 inhibits lung cancer bone\u2010specific metastasis by upregulating miR\u2010660\u20105p targeted 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