{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T10:16:34Z","timestamp":1785233794833,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773237"],"award-info":[{"award-number":["81773237"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study aimed to investigate the diagnostic and prognostic role of tumor\u2010educated leukocytes (TELs) mRNA in Chinese patients with non\u2010small cell lung cancer (NSCLC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The TELs collected underwent total RNA isolation. RNA\u2010sequencing (RNA\u2010seq) technology was used to analyze the transcriptome of the TELs. The mRNA expression levels of differential genes were analyzed by RT\u2010qPCR. Statistical analyses were performed using Prism and SPSS by Mann\u2013Whitney nonparametric test, Kruskal\u2010Wallis test and one\u2010way ANOVA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We used RNA\u2010seq technology to screen 95 differential genes (DEGs) from seven NSCLC and four controls, wherein 15 genes were upregulated, and 80 were downregulated. Of these, four genes were selected for further analysis, wherein one was upregulated (<jats:italic>GPX1<\/jats:italic>) and three were downregulated (<jats:italic>BCL9L<\/jats:italic>, <jats:italic>MAP3K7CL<\/jats:italic>, <jats:italic>PCSK7<\/jats:italic>). RT\u2010qPCR was performed in 431 samples (237 NSCLC, 194 healthy donors). The four\u2010gene panel showed significant differences (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) in the expression levels between NSCLC and healthy samples. ROC curves of the panel revealed an AUC of 0.803, with a sensitivity of 73.8% and specificity of 75.3%. <jats:italic>GPX1<\/jats:italic>, <jats:italic>BCL9L<\/jats:italic> and <jats:italic>PCSK7<\/jats:italic> genes distinguished early\u2010stage NSCLC patients from healthy group (<jats:italic>p<\/jats:italic> &lt;\u20090.05). When the three genes were combined to diagnose early\u2010stage NSCLC, the diagnostic efficacy was 0.772, sensitivity was 73.7%, and specificity was 72.2%. In addition, the downregulated gene <jats:italic>BCL9L<\/jats:italic> was associated with chemotherapeutic effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study provided a systematic description of gene expression profiling in the TELs. It is worth noting that these four genes may be potential candidate genes for NSCLC diagnostic biomarkers and provide a basis for further biological and functional studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.13833","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T08:52:39Z","timestamp":1611219159000},"page":"737-745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Tumor\u2010educated leukocytes <scp>mRNA<\/scp> as a diagnostic biomarker for non\u2010small cell lung cancer"],"prefix":"10.1111","volume":"12","author":[{"given":"Limin","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences  Jinan 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