{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:34:53Z","timestamp":1787160893003,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Thymic epithelial tumors (TETs) are relatively rare malignant thoracic tumors. Tumor mutation burden (TMB) and immune infiltration play important roles in tumorigenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Research data was obtained using the Cancer Genome Atlas (TCGA) database to evaluate the landscape of tumor mutations, related factors, and relationship of prognosis. The CIBERSORT algorithm was used to evaluate immune cell infiltration in TETs and its relationship with TMB. Immune\u2010related differentially expressed genes (irDEGs) were identified. Hub irDEGs independently related to prognosis were analyzed using univariate and multivariate Cox proportional hazard models. A survival signature was constructed from hub irDEGs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 122 patients were included in this study. GTF2I was the most common gene mutation. Higher TMB was significantly associated with the later stage, more advanced pathological type, and older age. The overall survival (OS) of patients in the low\u2010TMB group was significantly better. There was no significant correlation between TMB levels and PD\u2010L1 expression. Enrichment analysis showed that DEGs were mainly involved in the P13K\u2013Akt signaling pathway. There were significant differences in macrophage and other types of immune cell infiltration between the high\u2010 and low\u2010TMB groups. CCR5, FASLG, and CD79A independently relating to prognosis were screened from 391 irDEGs. The low\u2010risk group had a significantly better prognosis than the high\u2010risk group based on the signature, which has a good predictive effect on OS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In this study, TETs patients with high TMB had a significantly poor prognosis and an immune\u2010related gene signature was found to effectively evaluate the long\u2010term prognosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1759-7714.14002","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T09:29:30Z","timestamp":1621934970000},"page":"1995-2006","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Significance of tumor mutation burden and immune infiltration in thymic epithelial tumors"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7882-0047","authenticated-orcid":false,"given":"Zi\u2010Ming","family":"Wang","sequence":"first","affiliation":[{"name":"Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Department of Thoracic Surgery, Klinikum Ernst von Bergmann Academic Hospital of Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin  Potsdam Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi\u2010Rong","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Medicine Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Kaul","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Charit\u00e9\u2010Universit\u00e4tsmedizin Humboldt University Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Ismail","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Department of Thoracic Surgery, Klinikum Ernst von Bergmann Academic Hospital of Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin  Potsdam Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8947-666X","authenticated-orcid":false,"given":"Harun","family":"Badakhshi","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Berlin Germany"},{"name":"Department of Radiation Oncology, Klinikum Ernst von Bergmann Academic Hospital of Charit\u00e9 \u2010 Universit\u00e4tsmedizin Berlin  Potsdam Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11099"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semtcvs.2004.12.002"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-017-0424-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/esmoopen-2018-000442"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4971"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.3241"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-17-0386"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1406498"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdy495"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1713444"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(18)30062-7"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.77.3184"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-019-0202-8"},{"key":"e_1_2_6_15_1","first-page":"5459","article-title":"GTF2I mutations are common in thymic epithelial tumors but not in hematological malignancies","volume":"37","author":"Tarrini G","year":"2017","journal-title":"Anticancer Res."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2017.05.020"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.02.038"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2019.12.52"},{"key":"e_1_2_6_19_1","first-page":"153","article-title":"Oncogenic roles of the PI3K\/AKT\/mTOR Axis","volume":"407","author":"Aoki M","year":"2017","journal-title":"Curr Top Microbiol Immunol."},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-16-1080"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2011.03.023"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.126"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2012.11.018"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00172-9"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2010.06.003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.572"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri978"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1853"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2163544"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990219)84:1<74::AID-IJC14>3.0.CO;2-I"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56835-8"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-2684"}],"container-title":["Thoracic Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.14002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1759-7714.14002","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.14002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T06:37:50Z","timestamp":1693377470000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1759-7714.14002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,25]]},"references-count":31,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1111\/1759-7714.14002"],"URL":"https:\/\/doi.org\/10.1111\/1759-7714.14002","archive":["Portico"],"relation":{},"ISSN":["1759-7706","1759-7714"],"issn-type":[{"value":"1759-7706","type":"print"},{"value":"1759-7714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,25]]},"assertion":[{"value":"2021-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}