{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T18:50:18Z","timestamp":1763664618181,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T00:00:00Z","timestamp":1630886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972485"],"award-info":[{"award-number":["81972485"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>detection of anaplastic lymphoma receptor tyrosine kinase gene (ALK) rearrangements in patients with non\u2010small\u2010cell lung cancer (NSCLC) has become a routine pathological diagnosis worldwide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>there are three major conventional diagnostic methods for ALK fusions: fluorescent in situ hybridization (FISH); immunohistochemistry (Ventana IHC (D5F3)); and polymerase chain reaction (PCR). Next\u2010generation sequencing (NGS) technology as is a new tool for ALK status detection with great potential. These four methods are highly consistent in detecting ALK status (coincidence rate &gt;96%). However, discrepancies in ALK status have been found in some patients among these methods, which causes confusion for clinicians.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and conclusion<\/jats:title><jats:p>in this study, we analyzed two patients whose ALK statuses were not consistent using these four methods. We explored the potential reasons for deviation of the test results and found a novel EML4\u2010ALK break site, which had been not described previously.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.14123","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T06:36:07Z","timestamp":1630996567000},"page":"2773-2779","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A novel break site of <scp>EML4\u2010ALK<\/scp> report and a rare <scp>PRKAR1A\u2010ALK<\/scp> report analyzed by different <scp>ALK<\/scp> detection platforms in non\u2010small cell lung cancer patients"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9424-0520","authenticated-orcid":false,"given":"Xue","family":"Du","sequence":"first","affiliation":[{"name":"Beijing Institute of Radiation Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Fifth Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5277-4292","authenticated-orcid":false,"given":"Hongjun","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Lung Oncology Fifth Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhong","family":"Tai","sequence":"additional","affiliation":[{"name":"Department of Pathology Fifth Medical Center of Chinese PLA General Hospital  Beijing 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