{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:25:25Z","timestamp":1759742725461,"version":"3.40.5"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T00:00:00Z","timestamp":1649289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81730086"],"award-info":[{"award-number":["81730086"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To identify radio\u2010responsive genes and explore the biological function of encoded proteins in non\u2010small cell lung cancer (NSCLC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Radio\u2010responsive genes in irradiated H460 cells were screened from microarray data deposited in the Gene Expression Omnibus (GEO) database. A quantitative real time polymerase chain reaction assay was used to detect the expression of candidate radio\u2010responsive genes in irradiated cells. CCK\u20108 assay, EDU assay, clone formation assay, immunofluorescence and flow cytometry were conducted to evaluate the biological function of B cell translocation gene 2 (BTG2) in NSCLC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bioinformatic analysis using GES20549 showed that BTG2 was a radio\u2010responsive gene in irradiated H460 cells. The mRNA expression level of BTG2 was lower in H460 cells compared with that in BEAS\u20102B normal lung epithelial cells. BTG2 expression was elevated upon IR exposure, in a dose\u2010dependent but not a time\u2010dependent manner. CCK\u20108 and EDU assays revealed that BTG2 overexpression inhibited the growth rate of irradiated cells. Clone formation showed that elevated BTG2 promoted DNA damage of irradiated H460 cells. The number of \u03b3\u2010H2AX foci induced by DNA damage was also markedly increased upon BTG2 overexpression. Flow cytometry showed that BTG2 increased IR\u2010induced cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>BTG2 may be a novel radio\u2010responsive factor and a promising therapeutic target for radiotherapy of NSCLC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.14410","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T08:46:04Z","timestamp":1649321164000},"page":"1441-1448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Elevated <scp>BTG2<\/scp> improves the radiosensitivity of non\u2010small cell lung cancer (<scp>NSCLC<\/scp>) through apoptosis"],"prefix":"10.1111","volume":"13","author":[{"given":"Changchun","family":"Zhu","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2588-1855","authenticated-orcid":false,"given":"Aiying","family":"Xue","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5403-3698","authenticated-orcid":false,"given":"Guoxing","family":"Feng","sequence":"additional","affiliation":[{"name":"Tianjin Key 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