{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:30:44Z","timestamp":1747182644857,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As mesothelioma generally has an unfavorable prognosis, further advances are needed to improve the outcomes in patients with mesothelioma. In the present study, we generated and characterized a monoclonal antibody that could inhibit mesothelioma cell proliferation in a xenotransplantation mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We generated monoclonal antibodies by immunizing mice with cultured mesothelioma cells. These antibodies were then characterized by immunofluorescence staining, immunohistochemical staining, secondary antibody\u2010drug conjugate assay, antibody inoculation in a xenotransplantation mesothelioma mouse model, and mass spectrometry followed by small interfering RNA (siRNA) analysis. 5' rapid amplification of complementary DNA ends followed by sequencing was performed to deduce the amino acid sequences of the variable regions of the light and heavy chains of AX10.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An IgG2b \u03ba\u2010type AX10 antibody against the cell surface membrane of sarcomatoid mesothelioma cells was generated. AX10 immunoreactivity was detected in 12 out of 22 different mesothelioma tissue specimens, but there was little AX10 immunoreactivity in a normal human tissue array. AX10 decreased Matrigel invasion by MPM\u20101 cells but did not affect cell proliferation. Notably, AX10 significantly inhibited the proliferation of MPM\u20101 cells xenotransplanted into Severe combined immunodeficiency\u2010Nonobese diabetic mice. Matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight mass spectrometry followed by siRNA silencing indicated that AX10 reacted to a unique alternatively spliced isoform of sarcolemma\u2010associated protein. AX10 is composed of as yet unregistered amino acid sequences in its variable region.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>AX10 could have therapeutic potential for patients with sarcomatoid mesothelioma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.14591","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T16:37:50Z","timestamp":1659458270000},"page":"2566-2573","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tumor suppressor effect of an antibody on xenotransplanted sarcomatoid mesothelioma cells"],"prefix":"10.1111","volume":"13","author":[{"given":"Masayoshi","family":"Hasegawa","sequence":"first","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"},{"name":"Department of Pathology Matsunami General Hospital  Gifu 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