{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T19:27:51Z","timestamp":1772134071442,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T00:00:00Z","timestamp":1662595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The prognosis of thymic epithelial tumors (TETs) currently relies on the commonly adopted WHO classification and Masaoka staging system, which cannot reflect the undefined biological behaviors limiting them as prognostic factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we first identified 40 genes and 179 genes, respectively that were epigenetically upregulated and silenced, corresponding to a total of 509 functionally methylated CpG sites between thymomas and thymic carcinomas by using the TCGA dataset.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The methylation \u03b2\u2010values of cg20068620 in <jats:italic>MAPK4<\/jats:italic> and cg18770944 in <jats:italic>USP51<\/jats:italic> were significantly associated with recurrence\u2010free survival (RFS). In the independent validation cohort, only WHO classification and methylation \u03b2\u2010values of cg20068620 in <jats:italic>MAPK4<\/jats:italic> were independent prognostic factors for RFS in Chinese patients with TETs. A linear weighted model including these two factors was used to calculate the recurrence risk score (RRS). Time\u2010dependent ROC curve analysis revealed that RRS was overwhelmingly superior to WHO classification for predicting 3\u2010, 5\u2010, and 10\u2010year RFS and Masaoka stage for 3\u2010 and 5\u2010year RFS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggested that the methylation site cg20068620 in <jats:italic>MAPK4<\/jats:italic> can improve the accuracy of the WHO classification alone regarding the prognostic value of TETs recurrence.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.14628","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T08:49:57Z","timestamp":1662626997000},"page":"2844-2853","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Promotor methylation status of <scp>MAPK4<\/scp> is a novel epigenetic biomarker for prognosis of recurrence in patients with thymic epithelial tumors"],"prefix":"10.1111","volume":"13","author":[{"given":"Wei","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songlin","family":"Li","sequence":"additional","affiliation":[{"name":"Cancer Center, Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu China"},{"name":"Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhimin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Center, Renmin Hospital of Wuhan University  Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2298-1121","authenticated-orcid":false,"given":"He","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jungang","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizhao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0047-9560","authenticated-orcid":false,"given":"Chuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cancer Center, Daping Hospital Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11748-012-0119-y"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00595-005-3047-y"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2016.03.53"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0000000000000654"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0000000000000663"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00007312"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2020.01.14"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezv239"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13072-018-0205-1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-111314-035900"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.31155"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-019-0619-z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2011.09.022"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.75.5165"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.5958"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-0118-x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13061189"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2013.0072"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crad.2016.05.007"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2013.0073"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-0644"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-016-0144-8"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2008.07.015"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2019.10.018"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/1759-7714.13439"},{"issue":"10","key":"e_1_2_7_27_1","first-page":"2020","article-title":"USP51 promotes deubiquitination and stabilization of ZEB1","volume":"7","author":"Zhou ZC","year":"2017","journal-title":"Am J Cancer Res"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2021.12744"},{"issue":"1","key":"e_1_2_7_29_1","first-page":"315","article-title":"DNA methylation of GHSR, GNG4, HOXD9 and ASLL3 is a common epigenetic alteration in thymic carcinoma","volume":"56","author":"Kishibuchi R","year":"2020","journal-title":"Int J Oncol"}],"container-title":["Thoracic Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.14628","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1759-7714.14628","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.14628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T18:33:20Z","timestamp":1692642800000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1759-7714.14628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,8]]},"references-count":28,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/1759-7714.14628"],"URL":"https:\/\/doi.org\/10.1111\/1759-7714.14628","archive":["Portico"],"relation":{},"ISSN":["1759-7706","1759-7714"],"issn-type":[{"value":"1759-7706","type":"print"},{"value":"1759-7714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,8]]},"assertion":[{"value":"2022-06-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}