{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T23:42:08Z","timestamp":1789688528366,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Malignant mesothelioma (MM) is a rare tumor of mesothelial cells, with an increasing incidence both in developed and developing countries. MM has three major histological subtypes, in order of frequency, according to the World Health Organization (WHO) Classification of 2021: epithelioid, biphasic, and sarcomatoid MM. Distinction may be a challenging task for the pathologist, due to the unspecific morphology.<\/jats:p><jats:p>Here, we present two cases of diffuse MM subtypes to emphasize the immunohistochemical (IHC) differences, and to facilitate diagnostic difficulties. In our first case of epithelioid mesothelioma, the neoplastic cells showed cytokeratin 5\/6 (CK5\/6), calretinin, and Wilms\u2010tumor\u20101 (WT1) expression, while remaining negative with thyroid transcription factor\u20101 (TTF\u20101). BRCA1 associated protein\u20101 (BAP1) negativity was seen in the neoplastic cells' nucleus, reflecting loss of the tumor suppressor gene. In the second case of biphasic mesothelioma, expression of epithelial membrane antigen (EMA), CKAE1\/AE3, and mesothelin was observed, while WT1, BerEP4, CD141, TTF1, p63, CD31, calretinin, and BAP1 expressions were not detected.<\/jats:p><jats:p>Due to the absence of specific histological features, the differentiation between MM subtypes could be a challenging task. In routine diagnostic work, IHC may be the proper method in distinction. According to our results and literature data, CK5\/6, mesothelin, calretinin, and Ki\u201067 should be applied in subclassification.<\/jats:p>","DOI":"10.1111\/1759-7714.14827","type":"journal-article","created":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T01:38:44Z","timestamp":1676943524000},"page":"857-863","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Highlighting the immunohistochemical differences of malignant mesothelioma subtypes via case presentations"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9434-2989","authenticated-orcid":false,"given":"Anita","family":"Sejben","sequence":"first","affiliation":[{"name":"Department of Pathology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam\u00e1s","family":"Pancsa","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Tiszlavicz","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"Fur\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00f3ra","family":"Par\u00f3czai","sequence":"additional","affiliation":[{"name":"Department of Pulmonology University of Szeged  Deszk Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam\u00e1s","family":"Zombori","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00007014"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/environments6070076"},{"key":"e_1_2_11_4_1","first-page":"491","article-title":"Malignant pleural mesothelioma: an epidemiological perspective","volume":"1","author":"Robinson BM","year":"2012","journal-title":"Ann Cardiothorac Surg"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1693406"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017-0365-RA"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2017.10.09"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000495"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-3-34"},{"key":"e_1_2_11_10_1","first-page":"1433","article-title":"Malignant mesothelioma","volume":"29","author":"Ahmed I","year":"2013","journal-title":"Pak J Med Sci"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-021-03031-7"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2014.08.017"},{"key":"e_1_2_11_13_1","volume-title":"WHO Classification of Tumours Editorial Board\u2014Thoracic Tumours: WHO Classification of tumours","year":"2021"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002680020021"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2018.09.019"},{"key":"e_1_2_11_16_1","first-page":"70","article-title":"Mesothelioma: a review","volume":"12","author":"Mott FE","year":"2012","journal-title":"Ochsner J"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.15512.3"},{"key":"e_1_2_11_18_1","first-page":"364","article-title":"Sarcomatoid malignant mesothelioma: a clinicopathologic and immunohistochemical analysis of 22 cases","volume":"43","author":"Lao I","year":"2014","journal-title":"Zhonghua Bing Li Xue Za Zhi"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-2113.152665"},{"key":"e_1_2_11_20_1","first-page":"1","article-title":"Epidemiology and outcome of peritoneal and pleural mesothelioma subtypes in Norway. A 20 year nation\u2010wide study","volume":"27","author":"Brugustun OT","year":"2021","journal-title":"Acta Oncol"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12199-007-0017-6"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2017.10.125"},{"key":"e_1_2_11_23_1","first-page":"451","article-title":"Expression of glucose transporter protein 1 and desmin in reactive mesothelial hyperplasia and epithelioid mesothelioma","volume":"42","author":"Wei P","year":"2013","journal-title":"Zhonghua Bing Li Xue Za Zhi"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00920-9"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20071"},{"key":"e_1_2_11_26_1","first-page":"631","article-title":"Immunohistochemical profile of normal mesothelium and histocytic\/mesothelial hyerplasia: a case report","volume":"4","author":"Terada T","year":"2011","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_11_27_1","first-page":"12","article-title":"What's new in mesothelioma","volume":"110","author":"Ascoli V","year":"2018","journal-title":"Pathologica"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.134"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1310478"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-019-0310-0"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-021-03031-7"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2018-0457-OA"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr.2020.03.38"},{"key":"e_1_2_11_34_1","first-page":"14","article-title":"The immunohistochemical characterization of sarcomatoid malignant mesothelioma of the pleura","volume":"1","author":"Chirieac LR","year":"2011","journal-title":"Am J Cancer Res"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10112434"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017-0124-RA"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(97)80054-4"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017-0295-OA"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2018.04.023"}],"container-title":["Thoracic Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.14827","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1759-7714.14827","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.14827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T01:48:37Z","timestamp":1692668917000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1759-7714.14827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,20]]},"references-count":38,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1111\/1759-7714.14827"],"URL":"https:\/\/doi.org\/10.1111\/1759-7714.14827","archive":["Portico"],"relation":{},"ISSN":["1759-7706","1759-7714"],"issn-type":[{"value":"1759-7706","type":"print"},{"value":"1759-7714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,20]]},"assertion":[{"value":"2022-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}