{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T10:44:27Z","timestamp":1779273867959,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"26","license":[{"start":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T00:00:00Z","timestamp":1722729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017964","name":"Tianjin Municipal Health Commission","doi-asserted-by":"publisher","award":["ZC20013"],"award-info":[{"award-number":["ZC20013"]}],"id":[{"id":"10.13039\/100017964","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lung cancer is one of the major threats to human life worldwide. MiR\u2010190 has been found to perform essential roles in multiple cancer progression; however, there have been no studies focused on its function and underlying regulatory mechanism in lung cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The miR\u2010190 expression was detected by real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR). The cell functional experiments, including cell counting kit\u20108 (CCK\u20108), colony formation and transwell assay were conducted in vitro, as well as animal experiments performed in vivo. The regulation and potential binding sites of CBX4 on miR\u2010190 were predicted by TCGA data set and JASPAR website and verified by ChIP assay and dual\u2010luciferase reporter assay. The prospects binding site of miR\u2010190\u20103p on CBX4 3\u2032UTR region was predicted by StarBase and verified by dual\u2010luciferase reporter assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u2010190 was decreased in lung cancer cells. The overexpression of miR\u2010190 had no effects on cell proliferation, but significantly inhibited cancer metastasis both in vitro and in vivo. Moreover, miR\u2010190 expression could be transcriptionally inhibited by CBX4, and CBX4 was the direct target of miR\u2010190\u20103p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MiR\u2010190 served as a cancer metastasis inhibitor in lung cancer and formed a regulatory loop with CBX4. These findings provided emerging insights into therapeutic targets and strategies for metastatic lung cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.15415","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T03:33:57Z","timestamp":1722828837000},"page":"1889-1896","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CBX4<\/scp>\/<scp>miR<\/scp>\u2010190 regulatory loop inhibits lung cancer metastasis"],"prefix":"10.1111","volume":"15","author":[{"given":"Jian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Molecular Imaging and Nuclear Medicine Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer  Tianjin China"},{"name":"Key Laboratory of Cancer Prevention and Therapy Tianjin Tumor Hospital  Tianjin China"},{"name":"Tianjin's Clinical Research Center for Cancer Tianjin Tumor Hospital  Tianjin 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