{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T15:05:52Z","timestamp":1775919952363,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"29","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019346","name":"Instituto Nacional de Cancerolog\u00eda","doi-asserted-by":"publisher","award":["5P30CA014089\u201040"],"award-info":[{"award-number":["5P30CA014089\u201040"]}],"id":[{"id":"10.13039\/100019346","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lung cancer screening with low\u2010dose computed tomography (CT) scans (LDCT) has reduced mortality for patients with high\u2010risk smoking histories, but it has significant limitations: LDCT screening implementation remains low, high rates of false\u2010positive scans, and current guidelines exclude those without smoking histories. We sought to explore the utility of liquid biopsy (LBx) in early cancer screening and diagnosis of lung cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using the high\u2010definition single\u2010cell assay workflow, we analyzed 99 peripheral blood samples from three cohorts: normal donors (NDs) with no known pathology (<jats:italic>n<\/jats:italic>\u2009=\u200950), screening CT patients (<jats:italic>n<\/jats:italic>\u2009=\u200925) with Lung\u2010RADS score of 1\u20132, and biopsy (BX) patients (<jats:italic>n<\/jats:italic>\u2009=\u200924) with abnormal CT scans requiring tissue biopsy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For CT and BX patients, demographic information was roughly equivalent; however, average pack\u2010years smoked differed. A total of 14 (58%) BX patients were diagnosed with primary lung cancer (BX+). The comparison of the rare event enumerations among the cohorts revealed a greater incidence of total events, rare cells, and oncosomes, as well as specific cellular phenotypes in the CT and BX cohorts compared with the ND cohort. LBx analytes were also significantly elevated in the BX compared with the CT samples, but there was no difference between BX+ and BX\u2212 samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The data support the utility of the LBx in distinguishing patients with an alveolar lesion from those without, providing a potential avenue for prescreening before LDCT.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1759-7714.15429","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T06:28:52Z","timestamp":1725517732000},"page":"2100-2109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Circulation of rare events in the liquid biopsy for early detection of lung mass lesions"],"prefix":"10.1111","volume":"15","author":[{"given":"Karen","family":"Resnick","sequence":"first","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anya","family":"Shah","sequence":"additional","affiliation":[{"name":"Convergent Science Institute for Cancer, Michelson Center, University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Mason","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California  Los Angeles California USA"},{"name":"Convergent Science Institute for Cancer, Michelson Center, University of Southern California  Los Angeles California USA"},{"name":"Institute of Urology, Catherine &amp; Joseph Aresty Department of Urology Keck School of Medicine, University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2629-4505","authenticated-orcid":false,"given":"Peter","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California  Los Angeles California USA"},{"name":"Convergent Science Institute for Cancer, Michelson Center, University of Southern California  Los Angeles California USA"},{"name":"Institute of Urology, Catherine &amp; Joseph Aresty Department of Urology Keck School of Medicine, University of Southern California  Los Angeles California USA"},{"name":"Department of Biomedical Engineering Viterbi School of Engineering, University of Southern California  Los Angeles California USA"},{"name":"Department of Aerospace and Mechanical Engineering Viterbi School of Engineering, University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Nieva","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie N.","family":"Shishido","sequence":"additional","affiliation":[{"name":"Convergent Science Institute for Cancer, Michelson Center, University of Southern California  Los Angeles California 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