{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T18:14:38Z","timestamp":1784139278292,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["23K06423"],"award-info":[{"award-number":["23K06423"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K18396"],"award-info":[{"award-number":["24K18396"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Epithelial\u2010mesenchymal transition (EMT) plays a crucial role in carcinogenesis, including mesothelioma. D\u2010Meso\u2010Sonobe is a deciduoid\u2010type mesothelioma cell line with morphological features similar to those of epithelioid cells. Here, we report that D\u2010Meso\u2010Sonobe cells exhibit spindle cell mesenchymal features under continuous confluent culture conditions. The spindle cell mesenchymal D\u2010Meso\u2010Sonobe expresses zinc finger E\u2010box\u2010binding homeobox\u00a01 (Zeb1), which is a master regulator of EMT in the nucleus. Xenoplanted D\u2010Meso\u2010Sonobe cells expressed nuclear Zeb1 and yes\u2010associated protein at the cancer invasion front and focally expressed integrin subunit alpha V and actin alpha 2, which are molecular phenotypes acquired by EMT in mesothelioma. Subsequent RNA sequencing revealed that lysyl oxidase like 1 (LOXL1) was more highly expressed in cultured spindle mesenchymal D\u2010Meso\u2010Sonobe cells than in epithelioid cells. LOXL1 immunoreactivity was observed at the invasive front of xenoplanted D\u2010Meso\u2010Sonobe cells. The epithelial\u2010mesenchymal plasticity of D\u2010Meso\u2010Sonobe cells may be applicable for the development of candidate molecular agents targeting EMT in mesothelioma.<\/jats:p>","DOI":"10.1111\/1759-7714.70091","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T11:15:19Z","timestamp":1747826119000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Epithelial\u2010Mesenchymal Plasticity in the D\u2010Meso\u2010Sonobe Mesothelioma Cell Line: A Putative Model of Epithelial\u2013Mesenchymal Transition in Mesothelioma"],"prefix":"10.1111","volume":"16","author":[{"given":"Hiroshi","family":"Okubo","sequence":"first","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0181-1334","authenticated-orcid":false,"given":"Yuki","family":"Hanamatsu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"},{"name":"Center for One Medicine Innovative Translational Research; COMIT Gifu University  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1537-0093","authenticated-orcid":false,"given":"Chiemi","family":"Saigo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"},{"name":"Center for One Medicine Innovative Translational Research; COMIT Gifu University  Gifu Japan"},{"name":"The United Graduate School of Drug Discovery and Medical Information Sciences Gifu University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonobe","family":"Hiroshi","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology National Hospital Organization, Fukuyama Medical Center  Fukuyama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4264-3632","authenticated-orcid":false,"given":"Tamotsu","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"},{"name":"Center for One Medicine Innovative Translational Research; 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