{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:08:21Z","timestamp":1789769301686,"version":"4.0.1"},"reference-count":48,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7232066"],"award-info":[{"award-number":["7232066"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82200111"],"award-info":[{"award-number":["82200111"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Malignant pleural effusion (MPE) is a common complication in the advanced stage of cancer. High\u2010Fat Diet (HFD)\u2010induced obesity has become a common metabolic background in cancer patients. Recent studies have demonstrated that HFD induces gut dysbiosis, resulting in alterations in metabolites and immune responses. However, its role in MPE remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established an MPE mouse model under both normal chow and HFD conditions. Metagenomic sequencing of fecal samples and untargeted metabolomics of plasma were performed to assess alterations in gut microbiota and systemic metabolites, respectively. Bioinformatic and statistical analyses were conducted to identify significant microbial taxa and metabolic pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HFD\u2010fed mice exhibited increased pleural effusion. Metagenome data of the intestinal microbiome and metabolome profiles of plasma metabolites revealed key taxa\u2014\n                      <jats:italic>Akkermansiaceae<\/jats:italic>\n                      ,\n                      <jats:italic>Parabacteroides<\/jats:italic>\n                      , and\n                      <jats:italic>Muribaculaceae<\/jats:italic>\n                      \u2014as well as significant metabolic pathways involved in sphingolipid metabolism, glycerophospholipid metabolism, and steroid hormone biosynthesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings suggest that HFD may accelerate the MPE progression through modulation of gut microbiota and plasma metabolites, providing new strategies for prevention and treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1759-7714.70126","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T07:29:16Z","timestamp":1753082956000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metagenomic and Metabolomic Profiling Reveals the Impact of High\u2010Fat Diet on Malignant Pleural Effusion"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7312-8132","authenticated-orcid":false,"given":"Qing\u2010Yu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0225-4447","authenticated-orcid":false,"given":"Ming\u2010Ming","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital, Capital Medical University  Beijing China"},{"name":"Medical Research Center Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010Feng","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan\u2010Zhong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2822-5618","authenticated-orcid":false,"given":"Feng\u2010Shuang","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital, Capital Medical University  Beijing China"},{"name":"Medical Research Center Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1403503"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02649"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21114114"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2021.08.041"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.142"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.35980"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.918823"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.217096"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e23509"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.42"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010024\u201003211\u2010w"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010020\u201003175\u20105"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.217076"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252212223"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000133321.00456.00"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3010473"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2306776121"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11080482"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13568\u2010022\u201001428\u20104"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201004985\u20107"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnac072"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.1889109"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555\u2010023\u201010117\u2010y"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1711235114"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw7479"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu16162660"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0227886"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b01156"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12610"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2024.109424"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2007.05.020"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.4065\/mcp.2010.0099"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2024.103287"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.5604\/01.3001.0010.5501"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10081133"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010020\u201000443\u2010w"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944\u2010022\u201001713\u2010y"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201024\u20101611"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2012.01.003"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000492091"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.176304"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438\u2010025\u201002243\u20109"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010020\u20100375\u20103"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3028387"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2021.108890"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.6.6.821"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010021\u201000912\u20100"}],"container-title":["Thoracic Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.70126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:30:34Z","timestamp":1753867834000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1759-7714.70126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":48,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/1759-7714.70126"],"URL":"https:\/\/doi.org\/10.1111\/1759-7714.70126","archive":["Portico"],"relation":{},"ISSN":["1759-7706","1759-7714"],"issn-type":[{"value":"1759-7706","type":"print"},{"value":"1759-7714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70126"}}