{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T05:00:24Z","timestamp":1785387624078,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K06423"],"award-info":[{"award-number":["23K06423"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Interleukin 32 (IL32) has context\u2010dependent roles in carcinogenesis across cancer types. In this study, we examined IL32 in mesotheliomas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>IL32 protein expression was evaluated by immunohistochemistry in 56 mesothelioma tissue microarray specimens and by immunoblot analysis in cultured mesothelioma cell lines. The functional roles of IL32 were examined through ectopic expression of IL32\u03b2 and IL32\u03b8 isoforms in D\u2010Meso\u2010Sonobe cells with epithelial\u2010mesenchymal plasticity and through siRNA\u2010mediated IL32 downregulation followed by cell\u2010detachment\u2010induced apoptosis assays in epithelioid mesothelioma cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IL32 immunoreactivity was detected in 20 of 56 mesothelioma tissue specimens, including cytoplasmic staining in 20 of 39 epithelioid cases, whereas little or no immunoreactivity was found in 17 sarcomatoid cases. In immunoblot analysis, an IL32 protein band was detected in two cultured epithelioid mesothelioma cell lines (MPM\u20102 and TCC\u2010Meso\u20101), but not in sarcomatoid MPM\u20101 cells. Two IL32 isoforms, IL32\u03b2 and IL32\u03b8 cDNAs, were isolated from MPM\u20102 cells. Ectopic expression of IL32\u03b8, but not IL32\u03b2, inhibited the morphological transition from epithelioid to sarcomatoid features in D\u2010Meso\u2010Sonobe mesothelioma cells with mesothelial\u2010mesenchymal transition plasticity. Conversely, siRNA\u2010mediated downregulation of IL32 increased cell detachment\u2010induced apoptosis in MPM\u20102 and TCC\u2010Meso\u20101 epithelioid mesothelioma cells. Moreover, IL32 suppressed expression of secreted protein acidic and rich in cysteine, which is an epithelial\u2010mesenchymal transition\u2010related protein in mesothelioma cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings indicate that IL32 is expressed in epithelioid mesothelioma and may contribute to mesotheliomagenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1759-7714.70353","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T07:09:48Z","timestamp":1784099388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Interleukin 32 Expression in Mesothelioma"],"prefix":"10.1111","volume":"17","author":[{"given":"Takuya","family":"Mikamo","sequence":"first","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"},{"name":"Department of Pathology Matsunami General Hospital  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0118-4051","authenticated-orcid":false,"given":"Riko","family":"Niwa","sequence":"additional","affiliation":[{"name":"Department of Pathology Matsunami General Hospital  Gifu Japan"},{"name":"Center for Translational Research Matsunami General Hospital  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Hanamatsu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamotsu","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Translational Research Gifu University Graduate School of Medicine  Gifu Japan"},{"name":"Center for Translational Research Matsunami General Hospital  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00895-7"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00000811"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2018.09.019"},{"key":"e_1_2_11_5_1","first-page":"27","article-title":"Evaluation of Apoptosis and Immunohistochemical Expression of the Apoptosis\u2010Related Proteins in Mesothelioma","volume":"59","author":"Jin L.","year":"2010","journal-title":"Hiroshima Journal of Medical Sciences"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.32"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/resp.12874"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3MR0620-550R"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2019.52.3.019"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2023.14187"},{"issue":"1","key":"e_1_2_11_11_1","first-page":"E23","article-title":"IL\u201032\u03b3 Enhances TNF\u2010\u03b1\u2010Induced Cell Death in Colon Cancer","volume":"53","author":"Park E. 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