{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T08:29:15Z","timestamp":1786609755200,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pleural mesothelioma (PM) is an aggressive cancer with limited therapeutic options and poor prognosis, necessitating comprehensive model systems for mechanistic studies and drug discovery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Tumor cells were isolated from malignant pleural effusion of PM patients and subjected to primary culture and continuous passaging. Cell lines were successfully established after more than 40 continuous passages in\u00a0vitro. Cell identification included morphological analysis, species identification, short tandem repeat (STR) profiling, mycoplasma detection, in\u00a0vitro proliferation assays, in\u00a0vivo tumorigenicity testing in NOD\/SCID mice, and immunohistochemical characterization. Genomic landscapes and transcriptomic profiling were defined by whole\u2010exome, whole\u2010genome sequencing, and RNA\u2010sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Three Chinese\u2010derived pleural mesothelioma cell lines (PUMC\u2010MESO1, PUMC\u2010MESO3, and PUMC\u2010MESO4) were successfully established. All three cell lines exhibited epithelioid morphology with adherent growth patterns. Upon subcutaneous transplantation into NOD\/SCID mice, PUMC\u2010MESO1 and PUMC\u2010MESO4 demonstrated tumorigenicity, while PUMC\u2010MESO3 remained non\u2010tumorigenic after 3\u2009months of observation. Histopathological examination confirmed their mesothelioma origin through positive staining for mesothelial markers including calretinin, WT\u20101, and D2\u201040. Genomic analysis revealed characteristic PM genomic alterations including mutations in\n                      <jats:italic>BAP1<\/jats:italic>\n                      ,\n                      <jats:italic>NF2,<\/jats:italic>\n                      and\n                      <jats:italic>TP53<\/jats:italic>\n                      , along with\n                      <jats:italic>CDKN2A<\/jats:italic>\n                      deletions. Transcriptomic analyses revealed heterogeneous molecular features among the three cell lines. Although the PUMC\u2010MESO cell lines displayed distinct clustering patterns relative to the CCLE mesothelioma cohort, each retained transcriptomic similarities to specific established mesothelioma models. Drug sensitivity assays further demonstrated heterogeneous responses to standard therapeutic agents.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study reports the establishment and characterization of three novel PM cell lines. These models recapitulate key aspects of PM biology, exhibit diverse therapeutic responses, and provide a valuable new resource for investigating disease mechanisms and advancing precision oncology research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1759-7714.70366","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T11:42:58Z","timestamp":1785843778000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights Into Genomic Drivers, Transcriptomic Heterogeneity, and Therapeutic Vulnerabilities From Novel Mesothelioma Cell Lines"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9918-1845","authenticated-orcid":false,"given":"Yani","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences and School of Basic Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2319-3293","authenticated-orcid":false,"given":"Fang","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pathology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Peking University Cancer Hospital and Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayin","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences and School of Basic Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenli","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences and School of Basic Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Peking University Cancer Hospital and Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Peking University Cancer Hospital and Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1960-8465","authenticated-orcid":false,"given":"Xiaocui","family":"Bian","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences and School of Basic Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3720-4982","authenticated-orcid":false,"given":"Yuqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences and School of Basic Medicine Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2023.11.014"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2020-216602"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1121557"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(21)00043-6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09660-w"},{"key":"e_1_2_11_7_1","first-page":"890","article-title":"Experience in the Application of a New Cell Block Preparation Technology","volume":"48","author":"Hu S. S.","year":"2019","journal-title":"Zhonghua Bing Li Xue Za Zhi"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac382"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-18-0804"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.12.023"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.239244.118"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts034"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/kxj037"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtocrr.2024.100672"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO-24-02425"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2015.09.001"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3520"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2020-215027"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910440212"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0701-1"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28297-x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.11.136"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-2873"}],"container-title":["Thoracic Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.70366","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1759-7714.70366","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1759-7714.70366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T07:50:20Z","timestamp":1786607420000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1759-7714.70366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":23,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/1759-7714.70366"],"URL":"https:\/\/doi.org\/10.1111\/1759-7714.70366","archive":["Portico"],"relation":{},"ISSN":["1759-7706","1759-7714"],"issn-type":[{"value":"1759-7706","type":"print"},{"value":"1759-7714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70366"}}