{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T07:05:40Z","timestamp":1789455940375,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2023MH223"],"award-info":[{"award-number":["ZR2023MH223"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Thoracic Cancer"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pyroptosis\u2010related signaling contributes to lung adenocarcinoma (LUAD) progression and prognosis. Apigenin (AP) has anti\u2010inflammatory and cytoprotective properties, but its effects on pyroptosis\u2010associated signaling in injured LUAD cells remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A549 cells were treated with carbonyl cyanide\n                      <jats:italic>m<\/jats:italic>\n                      \u2010chlorophenyl hydrazone (CCCP) and ferrous sulfate (FeSO\n                      <jats:sub>4<\/jats:sub>\n                      ) to establish a cellular injury model. Cell morphology, Cell Counting Kit\u20108 signals, and pyroptosis\u2010associated proteins were assessed after AP treatment. Network pharmacology, molecular docking, molecular dynamics simulations, and analyses of The Cancer Genome Atlas and Gene Expression Omnibus datasets were used to prioritize candidate targets, characterize pyroptosis\u2010related genes, and develop a prognostic signature.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CCCP\/FeSO\n                      <jats:sub>4<\/jats:sub>\n                      reduced CCK\u20108 signals and induced morphological injury, whereas AP partially reversed these changes. AP also reduced alterations in GSDMD\u2010N, caspase\u20101, IL\u20101\u03b2, TNF\u2010\u03b1\/NF\u2010\u03baB pathway proteins, AIM2, and ASC. TNF was prioritized as a candidate target, and computational analyses predicted a potentially stable AP\u2013TNF interaction without demonstrating direct binding. Bioinformatic analyses identified distinct pyroptosis\u2010related LUAD subgroups and generated a prognostic signature that stratified survival in training and external validation cohorts. AIM2 was additionally prioritized as a LUAD\u2010 and stress\u2010associated candidate.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      AP attenuated CCCP\/FeSO\n                      <jats:sub>4<\/jats:sub>\n                      \u2010induced injury and pyroptosis\u2010associated molecular changes in A549 cells, potentially through TNF\u2010\u03b1\/NF\u2010\u03baB and AIM2\u2010related signaling. Further mechanistic validation is required.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1759-7714.70383","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:03:27Z","timestamp":1787835807000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Apigenin Alleviates Pyroptosis in Lung Adenocarcinoma Cells by Modulating\n                    <scp>TNF<\/scp>\n                    \u2010\u03b1\/\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    Signaling and\n                    <scp>AIM2<\/scp>\n                    Inflammasome Activation"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3374-1318","authenticated-orcid":false,"given":"Kairui","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenguo","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The Second Medical College of Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiwu","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1108-8047","authenticated-orcid":false,"given":"Chenyang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oncology Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong Medical and Pharmaceutical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University  Yantai Shandong 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