{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T20:17:18Z","timestamp":1768421838122,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T00:00:00Z","timestamp":1352332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2012,12]]},"abstract":"Earlier studies show that H<\/jats:styled-content>es1 expression is oscillatory in neural stem cells but sustained and high in the roof plate and the floor plate, and that such different dynamics of H<\/jats:styled-content>es1 expression (oscillatory versus sustained) regulate different proliferation and differentiation characteristics of these cells (active in neural stem cells but rather dormant in roof\/floor plate cells). The mechanism of how different dynamics of H<\/jats:styled-content>es1 expression is controlled remains to be determined. Here, we found that the seed sequence of microRNA<\/jats:styled-content>\u20109 (miR<\/jats:styled-content>\u20109) is complementary to the 3\u2032\u2010UTR<\/jats:styled-content> sequence of H<\/jats:styled-content>es1<\/jats:italic> mRNA<\/jats:styled-content>. MiR<\/jats:styled-content>\u20109 is highly expressed in the ventricular zone of the developing brain, which contains neural stem cells, but it is not expressed in the roof plate or the floor plate. Over\u2010expression of miR\u20109 negatively regulates the Hes1 protein expression by interacting with the 3\u2032\u2010UTR<\/jats:styled-content> of H<\/jats:styled-content>es1<\/jats:italic> mRNA<\/jats:styled-content>, thereby inducing cell cycle exit and neuronal differentiation. Conversely, knockdown of miR<\/jats:styled-content>\u20109 inhibits neuronal differentiation. Furthermore, knockdown of miR<\/jats:styled-content>\u20109 inhibits the oscillatory expression of H<\/jats:styled-content>es1<\/jats:italic> mRNA<\/jats:styled-content> in neural stem cells. These results indicate that miR<\/jats:styled-content>\u20109 regulates the proliferation and differentiation of neural stem cells by controlling the dynamics of H<\/jats:styled-content>es1 expression in the developing brain.<\/jats:p>","DOI":"10.1111\/gtc.12009","type":"journal-article","created":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T07:46:43Z","timestamp":1352360803000},"page":"952-961","source":"Crossref","is-referenced-by-count":104,"title":["MicroRNA<\/scp>9 regulates neural stem cell differentiation by controlling H<\/scp>es1 expression dynamics in the developing brain"],"prefix":"10.1111","volume":"17","author":[{"given":"Siok\u2010Lay","family":"Tan","sequence":"first","affiliation":[{"name":"Institute for Virus Research Kyoto University Kyoto 606\u20108507 Japan"},{"name":"Kyoto University Graduate School of Medicine Kyoto 606\u20108501 Japan"}]},{"given":"Toshiyuki","family":"Ohtsuka","sequence":"additional","affiliation":[{"name":"Institute for Virus Research Kyoto University Kyoto 606\u20108507 Japan"},{"name":"Kyoto University Graduate School of Medicine Kyoto 606\u20108501 Japan"},{"name":"Japan Science and Technology Agency CREST Shogoin\u2010Kawahara Sakyo\u2010ku Kyoto 606\u20108507 Japan"}]},{"given":"Aitor","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Institute for Virus Research Kyoto University Kyoto 606\u20108507 Japan"}]},{"given":"Ryoichiro","family":"Kageyama","sequence":"additional","affiliation":[{"name":"Institute for Virus Research Kyoto University Kyoto 606\u20108507 Japan"},{"name":"Kyoto University Graduate School of Medicine Kyoto 606\u20108501 Japan"},{"name":"Japan Science and Technology Agency CREST Shogoin\u2010Kawahara Sakyo\u2010ku Kyoto 606\u20108507 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