{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T18:57:34Z","timestamp":1725649054234},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T00:00:00Z","timestamp":1384387200000},"content-version":"vor","delay-in-days":4519,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>RD3\u20130028, a compound with a benzodithiin structure, was found to be a potent inhibitor of respiratory syncytial virus (RSV) replication. Its action is specific; no activity is seen against influenza A virus, measles virus, herpes simplex virus type 1 or 2, or human cytomegalovirus. A time\u2010dependent drug addition experiment indicated that the antiviral activity occurs in the late stage of the RSV replication cycle, since this compound completely inhibited syncytium formation even when added up to 16 hr after the infection of cell monolayers at an MOI of 3. RD3\u20130028 had no direct virucidal effect on RSV. Western blotting analysis showed that RD3\u20130028 significantly decreased the amount of RSV proteins released into the cell culture medium. Moreover, five independent isolates of the RSV long strain were selected for growth in RD3\u20130028 (5\u201320 \u03bcg\/ml). These resistant viruses were more than 80\u2010fold less sensitive to RD3\u20130028 than the long strain. The F gene segment of each of these viruses was sequenced and in each case the mutant RNA segment contained at least one sequence alteration, converting asparagine 276 to tyrosine (F1 protein). These results suggest that RD3\u20130028 inhibits RSV replication by interfering with intracellular processing of the RSV fusion protein, or a step immediately thereafter, leading to loss of infectivity.<\/jats:p>","DOI":"10.1111\/j.1348-0421.2001.tb02654.x","type":"journal-article","created":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T15:48:13Z","timestamp":1384444093000},"page":"531-537","source":"Crossref","is-referenced-by-count":15,"title":["Mechanism of Selective Inhibition of Respiratory Syncytial Virus by a Benzodithiin Compound (RD3\u20130028)"],"prefix":"10.1111","volume":"45","author":[{"given":"Kenji","family":"Sudo","sequence":"first","affiliation":[{"name":"Rational Drug Design Laboratories  Fukushima Fukushima 960\u20101242 Japan"},{"name":"Department of Microbiology Fukushima University School of Medicine  Fukushima Fukushima 960\u20101295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Konno","sequence":"additional","affiliation":[{"name":"Rational Drug Design Laboratories  Fukushima Fukushima 960\u20101242 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Rational Drug Design Laboratories  Fukushima Fukushima 960\u20101242 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Shigeta","sequence":"additional","affiliation":[{"name":"Department of Microbiology Fukushima University School of Medicine  Fukushima Fukushima 960\u20101295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Yokota","sequence":"additional","affiliation":[{"name":"Rational Drug Design Laboratories  Fukushima Fukushima 960\u20101242 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,14]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.1937"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.1.81"},{"key":"e_1_2_2_4_1","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1542\/peds.92.3.501","article-title":"Use of ribavirin in the treatment of respiratory syncytial virus infection","volume":"92","author":"Committee on Infectious Diseases, American Academy of Pediatrics","year":"1993","journal-title":"Pediatrics"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.4.1386"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-66-9-1983"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.7.1469"},{"key":"e_1_2_2_8_1","first-page":"1524","article-title":"The Respiratory Syncytial Virus Immune Globulin Study Group. 1993. 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