{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:53:37Z","timestamp":1761663217137},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T00:00:00Z","timestamp":1384387200000},"content-version":"vor","delay-in-days":2419,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A considerable number of patients infected with Hepatitis C virus subtype 1b (HCV\u20101b) do not respond to pegylated interferon\/ribavirin combination therapy. In this study we explored a useful factor(s) to predict treatment outcome. A total of 47 HCV\u20101b\u2010infected patients were treated with pegylated interferon\/ribavirin for 48 weeks. Sera of the patients were examined for the entire NS5A sequence of the HCV genome, HCV RNA titers and anti\u2010NS5A antibodies. According to their responses, the patients were divided into two groups, early viral responders who cleared the virus by week 16 (EVR[16w]) and those who did not (Non\u2010EVR[16w]). The mean number of mutations in the V3 region (aa 2356 to 2379) or that in the V3 region <jats:italic>plus<\/jats:italic> its N\u2010terminally flanking region, which we refer to as interferon\/ribavirin resistance\u2010determining region (IRRDR; aa 2334 to 2379), of NS5A obtained from the pretreatment sera was significantly larger for EVR(16w) compared with Non\u2010EVR(16w). Moreover, HCV\u20101b isolates with \u22655 mutations in V3 or those with \u22656 mutations in IRRDR were almost exclusively found in EVR(16w). Also, the presence of detectable levels of anti\u2010NS5A antibodies in the pretreatment sera was closely associated with EVR(16w). In conclusion, a high degree of sequence variation in V3 (\u22655) or IRRDR (\u22656) and the presence of detectable levels of anti\u2010NS5A antibodies in the pretreatment sera would be useful factors to predict EVR(16w). On the other hand, a less diverse sequence in V3 (\u22644) or IRRDR (\u22645) together with the absence of detectable anti\u2010NS5A antibodies could be a predictive factor for Non\u2010EVR(16w).<\/jats:p>","DOI":"10.1111\/j.1348-0421.2007.tb03922.x","type":"journal-article","created":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T14:05:19Z","timestamp":1384437919000},"page":"471-482","source":"Crossref","is-referenced-by-count":30,"title":["Prediction of Efficient Virological Response to Pegylated Interferon\/Ribavirin Combination Therapy by NS5A Sequences of Hepatitis C Virus and Anti\u2010NS5A Antibodies in Pre\u2010Treatment Sera"],"prefix":"10.1111","volume":"51","author":[{"given":"Ahmed","family":"El\u2010Shamy","sequence":"first","affiliation":[{"name":"Division of Microbiology Kobe University Graduate School of Medicine  Kobe Hyogo 650\u20100017 Japan"}]},{"given":"Mikiko","family":"Sasayama","sequence":"additional","affiliation":[{"name":"Division of Microbiology Kobe University Graduate School of Medicine  Kobe Hyogo 650\u20100017 Japan"}]},{"given":"Motoko","family":"Nagano\u2010Fujii","sequence":"additional","affiliation":[{"name":"Division of Microbiology Kobe University Graduate School of Medicine  Kobe Hyogo 650\u20100017 Japan"}]},{"given":"Noriko","family":"Sasase","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Kobe Asahi Hospital  Kobe Hyogo 653\u20100801 Japan"}]},{"given":"Susumu","family":"Imoto","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Kobe Asahi Hospital  Kobe Hyogo 653\u20100801 Japan"}]},{"given":"Soo Ryang","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Kobe Asahi Hospital  Kobe Hyogo 653\u20100801 Japan"}]},{"given":"Hak","family":"Hotta","sequence":"additional","affiliation":[{"name":"Division of Microbiology Kobe University Graduate School of Medicine  Kobe Hyogo 650\u20100017 Japan"}]}],"member":"311","published-online":{"date-parts":[[2013,11,14]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510250342"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199811193392102"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2003.50364"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-6-1373"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118025"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199601113340203"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-832925"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.04.009"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(98)80275-4"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510280630"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020047"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0197(98)00034-8"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8493"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2004.07.002"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21159"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510270529"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.36791"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200107053450107"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1086\/432760"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5424.110"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.3858-3864.2001"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)06102-5"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199811193392101"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2006.02863.x"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.05.019"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365549950161844"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015349924116"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2005.08.010"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2005.09.038"},{"key":"e_1_2_2_31_1","first-page":"1","article-title":"NIH Consens","volume":"19","author":"NIH Consensus Statement on Management of Hepatitis C","year":"2002","journal-title":"Stat. 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