{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T13:50:42Z","timestamp":1780062642680,"version":"3.54.0"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T00:00:00Z","timestamp":1157414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p>To isolate novel diagnostic markers and drug targets for pancreatic ductal adenocarcinoma (PDAC), we previously performed expression profile analysis of PDAC cells using a genome\u2010wide cDNA microarray combined with laser microdissection. Among dozens of up\u2010regulated genes identified in PDAC cells, we herein focused on one tyrosine kinase receptor, Eph receptor A4 (EphA4), as a molecular target for PDAC therapy. Immunohistochemical analysis validated EphA4 overexpression in approximately half of the PDAC tissues. To investigate its biological function in PDAC cells, we knocked down EphA4 expression by siRNA, which drastically attenuated PDAC cell viability. In concordance with the siRNA experiment, PDAC\u2010derivative cells that were designed to constitutively express exogenous EphA4 showed a more rapid growth rate than cells transfected with mock vector, suggesting a growth\u2010promoting effect of EphA4 on PDAC cells. Furthermore, the expression analysis for ephrin ligand family members indicated the coexistence of ephrinA3 ligand in PDAC cells with EphA4 receptor, and knockdown of ephrinA3 by siRNA also attenuated PDAC cell viability. These results suggest that the EphA4\u2013ephrinA3 pathway is likely to be a promising molecular target for pancreatic cancer therapy. (<jats:italic>Cancer Sci<\/jats:italic> 2006; 97: 1211\u20131216)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2006.00313.x","type":"journal-article","created":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T08:12:58Z","timestamp":1157443978000},"page":"1211-1216","source":"Crossref","is-referenced-by-count":53,"title":["EphA4 receptor, overexpressed in pancreatic ductal adenocarcinoma, promotes cancer cell growth"],"prefix":"10.1111","volume":"97","author":[{"given":"Megumi","family":"Iiizumi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayo","family":"Hosokawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Takehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyoun","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toyomasa","family":"Katagiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetoshi","family":"Eguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Ohigashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidewaki","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,9,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70298-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1177\/107327480401100204","article-title":"Surgical management of early\u2010stage pancreatic cancer","volume":"11","author":"Zervos EE","year":"2004","journal-title":"Cancer Control"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.56.2.106"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207392"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80500-0"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80276-7"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.21.1.309"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81240-4"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/20452"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1093703"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.9.1055"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63899-0"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207247"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-0143"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3667"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.026849"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2004.tb03194.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00007.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1158\/0008-5472.105.65.1","article-title":"Down\u2010regulation of RAB6KIFL\/KIF20A, a kinesin involved with membrane trafficking of discs large homologue 5, can attenuate growth of pancreatic cancer cell","volume":"65","author":"Taniuchi K","year":"2005","journal-title":"Cancer Res"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90434-D"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90177-F"},{"key":"e_1_2_6_23_2","first-page":"2840","article-title":"Antibody targeting of the EphA2 tyrosine kinase inhibits malignant cell behavior","volume":"62","author":"Kinch\u2010Carles K","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-1047"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0020"},{"key":"e_1_2_6_26_2","first-page":"5859","article-title":"Genome\u2010wide analysis of gene expression in synovial sarcomas using a cDNA microarray","volume":"62","author":"Nagayama S","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00625"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm856"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.20.5396"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2981-04.2004"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn994"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204004"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-3430-2"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/9.2.35"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11436"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1349-7006.2006.00313.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1349-7006.2006.00313.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T18:54:17Z","timestamp":1697396057000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1349-7006.2006.00313.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,5]]},"references-count":34,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1111\/j.1349-7006.2006.00313.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1349-7006.2006.00313.x","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"value":"1347-9032","type":"print"},{"value":"1349-7006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9,5]]}}}