{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T22:20:22Z","timestamp":1759702822120},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,3,12]],"date-time":"2008-03-12T00:00:00Z","timestamp":1205280000000},"content-version":"vor","delay-in-days":10146,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[1980,6]]},"abstract":"<jats:p><jats:bold>S<jats:sc>ummary<\/jats:sc><\/jats:bold> Crude, concentrated and isolated, labelled human factor IX was prepared and infused into animals. Initially, two dogs with severe haemophilia B received both preparations and factor IX clotting activity and label survived well. Kinetic parameters fit a two\u2010compartment open model.<\/jats:p><jats:p>For 12 infusions of concentrates into normal baboons, clotting and antigen data again fit the model. Similar kinetics, with less variability, were found for nine infusions of [<jats:sup>125<\/jats:sup>I]factor IX. Radioactivity of the first post\u2010infusion samples was &gt;90% precipitated in the double antibody system with an average of 84% of counts adsorbable to barium citrate. Compared to plasma, barium adsorbable counts gave a shorter <jats:italic>t<\/jats:italic><jats:sub>1\/2<\/jats:sub>\u03b2\u2010elimination phase suggesting a small recirculating <jats:sup>125<\/jats:sup>iodide pool. When [<jats:sup>125<\/jats:sup>I]factor IX was rechromatographed over heparin\u2010agarose, variable amounts of altered, labelled protein were present; this species was more rapidly cleared from plasma post\u2010infusion.<\/jats:p><jats:p>Twelve infusions of crude concentrates into baboons were given to animals whose platelets had been labelled with <jats:sup>51<\/jats:sup>Cr. Platelet counts as well as survival were then followed. For seven of these animals, mean counts decreased and the labelled platelets were destroyed, to an average of 21%, during the first 4 h post\u2010infusion. Subsequent platelet survival was normal. Three infusions of gel\u2010filtered, crude concentrates and two infusions of \u2018activated\u2019commercial concentrates did not significantly alter platelet survival.<\/jats:p>","DOI":"10.1111\/j.1365-2141.1980.tb07152.x","type":"journal-article","created":{"date-parts":[[2008,3,12]],"date-time":"2008-03-12T16:47:11Z","timestamp":1205340431000},"page":"329-342","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Human Factor IX in Animals: Kinetics from Isolated, Radiolabelled Protein and Platelet Destruction following Crude Concentrate Infusions"],"prefix":"10.1111","volume":"45","author":[{"given":"Arthur R.","family":"Thompson","sequence":"first","affiliation":[]},{"given":"Arden W.","family":"Forrey","sequence":"additional","affiliation":[]},{"given":"Patricia A.","family":"Gentry","sequence":"additional","affiliation":[]},{"given":"Kenneth J.","family":"Smith","sequence":"additional","affiliation":[]},{"given":"Laurence A.","family":"Harker","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,3,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.1961.tb00017.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1973.tb03534.x"},{"key":"e_1_2_1_4_1","first-page":"110","volume-title":"The Treatment of Haemophilia A and B and von Willebrand's Disease","author":"Biggs R.","year":"1978"},{"key":"e_1_2_1_5_1","first-page":"127","volume-title":"The Treatment of Haemophilia A and B and von Willebrand's Disease","author":"Biggs R.","year":"1978"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1978.tb03635.x"},{"key":"e_1_2_1_7_1","volume-title":"Computational Handbook of Statistics","author":"Bruning J.L.","year":"1968"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1978.tb02907.x"},{"key":"e_1_2_1_9_1","first-page":"266","article-title":"Pharmacokinetics of human factor IX infused into normal baboons","volume":"50","author":"Forrey A.W.","year":"1977","journal-title":"Blood"},{"key":"e_1_2_1_10_1","first-page":"162","article-title":"Pharmacokinetics of human factor IX in a dog with severe hemophilia B","volume":"38","author":"Gentry P.A.","year":"1977","journal-title":"Thrombosis and Haemostasis"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197510022931406"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109494"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1055\/s-0038-1647933","article-title":"Various prothrombin complex concentrates and their effect on coagulation and fibrinolysis in vivo","volume":"35","author":"Hedner U.","year":"1976","journal-title":"Thrombosis and Haemostasis"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196903132801103"},{"key":"e_1_2_1_15_1","first-page":"640","article-title":"Thromboembolic complications","volume":"33","author":"Kasper C.K.","year":"1975","journal-title":"Thrombosis et Diatheses Haemorrhagica"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1647857"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-4809(70)90045-5"},{"key":"e_1_2_1_18_1","first-page":"600","article-title":"Factor IX concentrates","volume":"33","author":"M\u00e9nach\u00e9 D.","year":"1975","journal-title":"Thrombosis et Diatheses Haemorrhagica"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"5946","DOI":"10.1016\/S0021-9258(17)34560-X","article-title":"Human blood coagulation factor IX. Purification, properties, and mechanism of activation by activated factor IX","volume":"253","author":"\u00d8sterud B.","year":"1978","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-708X(60)90141-1"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V53.5.828.828"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1976.16677060245.x"},{"key":"e_1_2_1_23_1","first-page":"307A","article-title":"Kinetics of 125I\u2010human factor IX (IX) in a patient with hemophilia B. (Abstract)","volume":"27","author":"Smith K.J.","year":"1979","journal-title":"Clinical Research"},{"key":"e_1_2_1_24_1","first-page":"1","article-title":"Preparation and properties of a therapeutic factor IX concentrate","volume":"32","author":"Suomela H.","year":"1977","journal-title":"Vox Sanguinis"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108712"},{"key":"e_1_2_1_26_1","first-page":"922","article-title":"Removal of heparin and protamine from plasma","volume":"88","author":"Thompson A.R.","year":"1976","journal-title":"Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00613a030"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1978.tb01039.x"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-197705000-00003"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2141.1980.tb07152.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2141.1980.tb07152.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T16:44:32Z","timestamp":1699721072000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2141.1980.tb07152.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,6]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,6]]}},"alternative-id":["10.1111\/j.1365-2141.1980.tb07152.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2141.1980.tb07152.x","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,6]]},"assertion":[{"value":"2008-03-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}