{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:22:49Z","timestamp":1774563769263,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T00:00:00Z","timestamp":1146096000000},"content-version":"vor","delay-in-days":5291,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[1991,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Using the technique of <jats:italic>in situ<\/jats:italic> hybridization, we have attempted to identify messenger RNA for tumour necrosis factor\u2010alpha (TNF\u03b1) in cells infiltrating allergen\u2010induced late phase reaetion (LPR) of the skin and the nose ofatopic subjects. We have also compared the number of TNF\u03b1 mRNA positive cells in bronchoalveolar lavage (BAL) from atopic asthmatics and normal controls. Twenty\u2010four hours after local allergen challenge, 12\/14 skin biopsies and 9\/10 nasal biopsies had positive hybridization signals for TNF\u03b1 mRNA whereas only 4\/14 and 2\/10 biopsies were positive in the relevant diluent controls. Compared with diluent sites significantly increased numbers of cells expressing mRNA for TNF\u03b1 were observed in the LPR of skin (P &lt;0.004) and nose(P &lt;0.006). All BAL from asthmatics (n= 10) and from normal volunteers (<jats:italic>n<\/jats:italic>= 10) had cells showing positive hybridization signals for TNF\u03b1 mRN A but these were at increased frequency in asthmatics (P&lt; 0.001). These results suggest that TNF\u03b1 may be an important cytokine in atopic allergic inflammation.<\/jats:p>","DOI":"10.1111\/j.1365-2222.1991.tb03205.x","type":"journal-article","created":{"date-parts":[[2006,4,28]],"date-time":"2006-04-28T13:40:15Z","timestamp":1146231615000},"page":"745-750","source":"Crossref","is-referenced-by-count":151,"title":["TNF\u03b1 mRNA expression in allergic inflammation"],"prefix":"10.1111","volume":"21","author":[{"given":"SUN","family":"YING","sequence":"first","affiliation":[]},{"given":"D. S.","family":"ROBINSON","sequence":"additional","affiliation":[]},{"given":"V.","family":"VARNEY","sequence":"additional","affiliation":[]},{"given":"QIU","family":"MENG","sequence":"additional","affiliation":[]},{"given":"A.","family":"TSICOPOULOS","sequence":"additional","affiliation":[]},{"given":"R.","family":"MOQBEL","sequence":"additional","affiliation":[]},{"given":"S. R.","family":"DURHAM","sequence":"additional","affiliation":[]},{"given":"A. 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