{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T06:58:07Z","timestamp":1775977087284,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":12566,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[1974,2]]},"abstract":"<jats:p><jats:bold>Abstract. <\/jats:bold> The effect of colchicine upon glucose\u2010induced insulin release by the isolated perfused rat pancreas was examined in order to characterize the participation of the \u03b2\u2010cell microtubular apparatus in the biphasic insulin secretory response to glucose. After a short pretreatment (25 min.) with a low colchicine concentration (2.10<jats:sup>\u20105<\/jats:sup>M), both the early and late phases of glucose\u2010induced insulin secretion were markedly enhanced. As either the concentration of colchicine or the length of the pretreatment period with this mitotic spindle\u2010inhibitor were increased, the facilitating effect faded out and partial inhibition of insulin release was observed. However, colchicine, even at high concentration, markedly enhanced the residual early release of insulin evoked by glucose in the presence of diazoxide (0.05 mg\/ml). These findings suggest that (i) a fraction of the early phase of insulin secretion completely escapes from the inhibitory effect of colchicine and may correspond, therefore, to a modality of hormonal release which does not involve the oriented intracellular translocation of secretory granules; and (ii) extensive disruption of the micro\u2010tubular apparatus is associated with inhibition of insulin release, especially during the late phase of the secretory response to glucose.<\/jats:p>","DOI":"10.1111\/j.1365-2362.1974.tb00407.x","type":"journal-article","created":{"date-parts":[[2008,3,20]],"date-time":"2008-03-20T15:20:21Z","timestamp":1206026421000},"page":"299-305","source":"Crossref","is-referenced-by-count":19,"title":["Dynamics of Insulin Release and Microtubular\u2010Microfilamentous System"],"prefix":"10.1111","volume":"4","author":[{"given":"G.","family":"Somers","sequence":"first","affiliation":[]},{"given":"E. Van","family":"Obberghen","sequence":"additional","affiliation":[]},{"given":"G.","family":"Devis","sequence":"additional","affiliation":[]},{"given":"M.","family":"Ravazzola","sequence":"additional","affiliation":[]},{"given":"F.","family":"Malaisse\u2010Lagae","sequence":"additional","affiliation":[]},{"given":"W. J.","family":"Malaisse","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00421414"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/2191177a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.49.2.530"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.20.5.257"},{"key":"e_1_2_1_5_2","first-page":"28","article-title":"Dual effect of colchicine upon the dynamics of insulin release by the isolated and perfused rat pancreas (abstract). Excerpta med","volume":"280","author":"Obberghen E.","year":"1973","journal-title":"Int. Congr. Ser."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107269"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(66)90089-8"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)74360-3"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-81-2-226"},{"key":"e_1_2_1_10_2","first-page":"583","article-title":"The Pilot gland approach to the study of insulin secretory dynamics","volume":"27","author":"Bergman R. N.","year":"1971","journal-title":"Recent Progr. Hormone Res."},{"key":"e_1_2_1_11_2","first-page":"25","article-title":"Comparative effect of di\u2010phenylhydantoin and diazoxide upon biphasic insulin secretion from the isolated perfused rat pancreas, with computerized correlation of biologic responses (abstract). Excerpta med","volume":"280","author":"Levin S. 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Chem."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(73)80625-8"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1974.tb02350.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1974.tb00409.x"}],"container-title":["European Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2362.1974.tb00407.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2362.1974.tb00407.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T00:24:38Z","timestamp":1699662278000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2362.1974.tb00407.x"}},"subtitle":["III. Effect of Colchicine upon Glucose\u2010Induced Insulin Secretion"],"short-title":[],"issued":{"date-parts":[[1974,2]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1974,2]]}},"alternative-id":["10.1111\/j.1365-2362.1974.tb00407.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2362.1974.tb00407.x","archive":["Portico"],"relation":{},"ISSN":["0014-2972","1365-2362"],"issn-type":[{"value":"0014-2972","type":"print"},{"value":"1365-2362","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,2]]}}}