{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:35:34Z","timestamp":1773290134229,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,11,4]],"date-time":"2004-11-04T00:00:00Z","timestamp":1099526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes to Cells"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p>The human XPV (xeroderma pigmentosum variant) gene is responsible for the cancer\u2013prone xeroderma pigmentosum syndrome and encodes DNA polymerase \u03b7 (pol \u03b7), which catalyses efficient translesion synthesis past <jats:italic>cis<\/jats:italic>\u2010syn cyclobutane thymine dimers (TT dimers) and other lesions. The fidelity of DNA synthesis by pol \u03b7 on undamaged templates is extremely low, suggesting that pol \u03b7 activity must be restricted to damaged sites on DNA. Little is known, however, about how the activity of pol \u03b7 is targeted and restricted to damaged DNA. Here we show that pol \u03b7 binds template\/primer DNAs regardless of the presence of TT dimers. Rather, enhanced binding to template\/primer DNAs containing TT dimers is only observed when the 3\u2032\u2010end of the primer is an adenosine residue situated opposite the lesion. When two nucleotides have been incorporated into the primer beyond the TT dimer position, the pol \u03b7\u2010template\/primer DNA complex is destabilized, allowing DNA synthesis by DNA polymerases \u03b1 or \u03b4 to resume. Our study provides mechanistic explanations for polymerase switching at TT dimer sites.<\/jats:p>","DOI":"10.1111\/j.1365-2443.2004.00797.x","type":"journal-article","created":{"date-parts":[[2004,11,25]],"date-time":"2004-11-25T12:25:27Z","timestamp":1101385527000},"page":"1139-1150","source":"Crossref","is-referenced-by-count":63,"title":["DNA binding properties of human DNA polymerase \u03b7: implications for fidelity and polymerase switching of translesion 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