{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:57:34Z","timestamp":1758635854807},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T00:00:00Z","timestamp":1159488000000},"content-version":"vor","delay-in-days":4807,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[1993,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The compartmental interrelationships of the metabolically related amino\u2010acids glutamate, GABA and glutamine and the metabolically unrelated amino\u2010acids taurine and glycine in the rodent pituitary, were investigated by light microscopic immunocytochemistry using highly specific antisera. Glutamate\u2010like immunoreactivity was abundant in astrocytes in the posterior pituitary. Glutamine immunoreactivity was present only at low levels in the posterior pituitary, but was abundant in astrocytes within the intermediate lobe. Other glia\u2010like ceils in the anterior pituitary were also glutamine\u2010immunoreactive. GABA immunoreactivity was abundant in the intermediate lobe but absent from anterior and posterior lobes. The GABA immunoreactivity mainly took the form of small punctata, the majority of which were in intimate apposition to the glutamine\u2010immunoreactive glia. Strong taurine immunoreactivity was present in astrocytes in the posterior pituitary but only weak labelling was present in intermediate and anterior lobes of the pituitary. Specific glycine immunoreactivity was not detected in the pituitary. These results suggest that glutamate\u2010immunoreactive astrocytes in the posterior pituitary, unlike glia in loci such as the retina, do not convert much, if any, of their glutamate content into glutamine (or if they do, it is rapidly further metabolized to another compound), whereas those astrocytes in the intermediate lobe do contain glutamine. The spatial association of GABAergic fibres with glutamine\u2010positive astrocytes raises the possibility that astrocytes in the intermediate lobe receive a GABAergic innervation. Glutamate, glutamine and taurine (or their metabolites) may have roles as neuroactive substances regulating pituitary secretion.<\/jats:p>","DOI":"10.1111\/j.1365-2826.1993.tb00494.x","type":"journal-article","created":{"date-parts":[[2006,9,29]],"date-time":"2006-09-29T10:30:39Z","timestamp":1159525839000},"page":"349-356","source":"Crossref","is-referenced-by-count":34,"title":["Immunocytochemistry of Amino\u2010Acids in the Rodent Pituitary Using Extremely Specific, Very High Titre Antisera"],"prefix":"10.1111","volume":"5","author":[{"given":"D. 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