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Excisive recombination requires two transposon\u2010encoded proteins designated Xis\u2010Tn and Int\u2010Tn. We have shown that, following excision, Tn1545 is a circular structure with ends separated by either of the two hexanucleotides that were present at the transposon\u2010target junctions. Using a <jats:italic>trans<\/jats:italic>\u2010complementation assay, we have demonstrated that Int\u2010Tn is able to catalyse <jats:italic>in vivo<\/jats:italic> integration of a circular intermediate of Tn1545 defective for integration and excision. Comparison of integration sites suggests that limited sequence homology at the vicinity of the recombining sites is required for integration of the element. These data support the hypothesis that the integration\/excision systems of conjugative transposons from Grampositive cocci and of lambdoid phages from Gramnegative bacilli have evolved from a common ancestor.<\/jats:p>","DOI":"10.1111\/j.1365-2958.1990.tb02062.x","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T17:37:32Z","timestamp":1161970652000},"page":"1513-1521","source":"Crossref","is-referenced-by-count":76,"title":["The integration\u2010excision system of the conjugative transposon Tn 1545 is structurally and functionally related to those of lambdoid 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