{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:53:53Z","timestamp":1778028833698,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T00:00:00Z","timestamp":1161907200000},"content-version":"vor","delay-in-days":4896,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[1993,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Using we11\u2010characterized mutant host cell lines, deficient in specific enzymes of energy and nucleotide metabolism, we addressed numerous questions regarding nucleotide metabolism in the obligate intracellular bacterium <jats:italic>Chlamydia trachomatis.<\/jats:italic> The results presented indicate that <jats:italic>C. trachomatis<\/jats:italic>: (i) does not absolutely depend on mitochondrial generated ATP for survival; (ii) does have a significant draw on host\u2010cell NTP pools but does not have a detrimental effect on the ability of the host cell to maintain its energy charge; (iii) lacks the ability to synthesize purine and pyrimidine nucleotides <jats:italic>de novo<\/jats:italic>; (iv) is not capable of interconverting purine nucleotides; and (v) possesses the pyrimidine metabolic\u2010pathway enzymes CTP synthetase and deoxycytidine nucleotide deaminase. In total our results indicate that <jats:italic>C. trachomatis<\/jats:italic> is auxotrophic for host\u2010cell ATP, GTP and UTP. In contrast, CTP can be obtained from the host cell or it can be synthesized from UTP by the parasite.<\/jats:p>","DOI":"10.1111\/j.1365-2958.1993.tb01655.x","type":"journal-article","created":{"date-parts":[[2006,10,28]],"date-time":"2006-10-28T01:34:51Z","timestamp":1161999291000},"page":"1105-1114","source":"Crossref","is-referenced-by-count":85,"title":["The obligate intracellular bacterium <i>Chlamydia trachomatis<\/i> is auxotrophic for three of the four ribonucleoside triphosphates"],"prefix":"10.1111","volume":"8","author":[{"given":"Graham","family":"Tipples","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant","family":"McClarty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00851a033"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"21087","DOI":"10.1016\/S0021-9258(19)30049-3","article-title":"Purine deoxynucleoside salvage in Giardia lamblia","volume":"264","author":"Baum K.F.","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.1.2.171"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"2742","DOI":"10.1128\/jb.174.9.2742-2747.1992","article-title":"Construction of physical and genetic maps of C. trachomatis serovar L2 by pulsed\u2010field gel electrophoresis","volume":"174","author":"Birkelund S.","year":"1992","journal-title":"J Bacteriol"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0620315"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.31.3.1161-1176.1981"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1128\/iai.25.1.98-102.1979","article-title":"Use of HeLa cell guanine nucleotides by Chlamydia psittaci","volume":"25","author":"Cellabos M.M.","year":"1979","journal-title":"Infect Immun"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01538838"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.173.21.6670-6677.1991"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.me.39.020188.002041"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(79)80005-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1139\/m70-182"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1988.tb02708.x-i1"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1128\/jb.122.2.393-400.1975","article-title":"Utilization of L\u2010cell nucleoside triphosphates by Chlamydia psittaci for ribonucleic add synthesis","volume":"122","author":"Hatch T.P.","year":"1975","journal-title":"J Bacteriol"},{"key":"e_1_2_1_16_1","first-page":"105","volume-title":"Microbiology of Chlamydia","author":"Hatch T.P.","year":"1988"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1128\/jb.150.2.662-670.1982","article-title":"Adenine nudcleotide and lysine transport in Chlamydia psittaci","volume":"150","author":"Hatch T.P.","year":"1982","journal-title":"J Bacteriol"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01534400"},{"key":"e_1_2_1_19_1","first-page":"24","volume-title":"Microbiology of Chlamydia","author":"Matsumoto A.","year":"1988"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.16.4922-4931.1991"},{"key":"e_1_2_1_21_1","first-page":"149","volume-title":"Metabolism of Nucleotides. 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