{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T13:46:23Z","timestamp":1767275183605},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T00:00:00Z","timestamp":1161907200000},"content-version":"vor","delay-in-days":4682,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Rhizobium<\/jats:italic> species elicit the formation of nitrogen\u2010fixing root nodules through a complex interaction between bacteria and plants. Various bacterial genes involved in the nodulation and nitrogen\u2010fixation processes have been described and most have been localized on the symbiotic plasmids (pSym). We have found a gene encoding citrate synthase on the pSym plasmid of <jats:italic>Rhizobium tropici<\/jats:italic>, a species that forms nitrogen\u2010fixing nodules on the roots of beans <jats:italic>(PhasBoius vuigaris)<\/jats:italic> and trees <jats:italic>(Leucaena<\/jats:italic> spp.). Citrate synthase is a key metabolic enzyme that incorporates carbon into the tricarboxylic acid cycle by catalysing the condensation of acetyl\u2010CoA and oxalo\u2010acetic acid to form citrate. <jats:italic>R. tropici pcsA<\/jats:italic> (the plasmid citrate synthase gene) is closely related to the corresponding genes of Proteobacteria. <jats:italic>pcsA<\/jats:italic> inactivation by a <jats:italic>Tn5\u2010mob<\/jats:italic> insertion causes the bacteria to form fewer nodules (30\u201350% of the original strain) and to have a decreased citrate synthase activity in minimal medium with sucrose. A clone carrying the pcsA gene complemented ail the phenotypic alterations of the <jats:italic>pcsA<\/jats:italic> mutant, and conferred <jats:italic>Rhizobium iegumino\u2010sarum<\/jats:italic> bv. <jats:italic>phaseoli<\/jats:italic> (which naturally lacks a plasmid citrate synthase gene) a higher nodulation and growth capacity in correlation with a higher citrate synthase activity. We have also found that <jats:italic>pcsA<\/jats:italic> gene expression is sensitive to iron availability, suggesting a possible role of <jats:italic>pcsA<\/jats:italic> in iron uptake.<\/jats:p>","DOI":"10.1111\/j.1365-2958.1994.tb00311.x","type":"journal-article","created":{"date-parts":[[2006,10,28]],"date-time":"2006-10-28T01:59:20Z","timestamp":1162000760000},"page":"315-321","source":"Crossref","is-referenced-by-count":41,"title":["Nodulating ability of <i>Rhizobium tropici<\/i> is conditioned by a plasmid\u2010encoded citrate synthase"],"prefix":"10.1111","volume":"11","author":[{"given":"Marco A.","family":"Pardo","sequence":"first","affiliation":[]},{"given":"Jaime","family":"Lagunez","sequence":"additional","affiliation":[]},{"given":"Juan","family":"Miranda","sequence":"additional","affiliation":[]},{"given":"Esperanza","family":"Martinez","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,10,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00308a008"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.142.2.581-587.1980"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00331029"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.10.5542-5550.1989"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1128\/jb.155.2.616-622.1983","article-title":"Iron uptake from ferrichrome A and iron citrate in Ustilago sphaerogena","volume":"155","author":"Ecker D.J.","year":"1983","journal-title":"J Bacteriol"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(78)90016-1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00390804"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.155.3760.279"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.4.2096-2104.1990"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.6.3298-3303.1990"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90462-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1981.tb06357.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02667740"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02730.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01535.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1128\/aem.56.8.2384-2388.1990","article-title":"Increased bean (Phaseoius vuigaris L.) nodulation competitiveness of genetically modified Rhizobium strains","volume":"56","author":"Martinez\u2010Romero E.","year":"1990","journal-title":"Appl Environm Microbiol"},{"key":"e_1_2_1_19_1","first-page":"1779","article-title":"Reiteration of nitrogen fixation gene sequences and specificity of Rhizobium in nodulation and nitrogen fixation in Phaseoius vuigaris","volume":"131","author":"Mart\u00ednez E.","year":"1985","journal-title":"J Gen Microbiol"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.6.2828-2834.1987"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-3-417"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.149.1.131-135.1982"},{"key":"e_1_2_1_23_1","first-page":"3","volume-title":"Iron Transport in Microbes. 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