{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T10:42:52Z","timestamp":1774780972216,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T00:00:00Z","timestamp":1161907200000},"content-version":"vor","delay-in-days":4531,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To Identify elements participating In the process of transformation, a bank of genetically altered mutants of Streptococcus pneumoniae with defects in exported proteins was assessed for a decrease in transformation efficiency. One mutant consistentiy transformed 10\u2010foid less than the parent strain. Sequence analysis and reconstitution of the altered locus revealed a gene, <jats:italic>plpA<\/jats:italic> (permease\u2010like protein), which encodes a putative substrate\u2010binding protein belonging to the family of bacterial permeases responsible for peptide transport. The derived amino acid sequence for this gene was 80% similar to AmiA, a peptide\u2010binding protein homologue from pneumococcus, and 50% similar over 230 amino acids to SpooKA which is a regulatory element in the process of transformation and sporulation in <jats:italic>Bacillus subtilis.<\/jats:italic> PIpA fusions to alkaline phosphatase (PhoA) were shown to be membrane associated and labelled with [<jats:sup>3<\/jats:sup>H]\u2010palmitic acid, which probably serves as a membrane anchor. Experiments designed to define the roles of the pIpA and ami determinants in the process of transformation showed that: (i) mutants with defects in plpA were &gt;90% transformation deficient while ami mutants exhibited up to a fourfold increase in transformation efficiency; (ii) compared to the parental strain, the onset of competence in an ami mutant occurred earlier in logarithmic growth, whereas the onset was delayed in a plpA mutant; and (ill) the plpA mutation decreases the expression of a competence\u2010regulated locus. Since the permease mutants would fail to bind specific ligands, it seems likely that the substrate\u2010permease interaction modulates the process of transformation.<\/jats:p>","DOI":"10.1111\/j.1365-2958.1994.tb01076.x","type":"journal-article","created":{"date-parts":[[2006,10,28]],"date-time":"2006-10-28T02:24:34Z","timestamp":1162002274000},"page":"881-892","source":"Crossref","is-referenced-by-count":53,"title":["Peptide permeases modulate transformation in <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1111","volume":"12","author":[{"given":"B. J.","family":"Pearce","sequence":"first","affiliation":[]},{"given":"A. M.","family":"Naughton","sequence":"additional","affiliation":[]},{"given":"H. 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