{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T14:41:41Z","timestamp":1772203301752,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Toxoplasma gondii<\/jats:italic> sporozoites possess an array of stage\u2010specific antigens that are localized to the membrane and internal cellular space, as well as secreted into the primary parasitophorous vacuole. Specific labelling of viable sporozoites excysted from oocysts reveals a complex admixture of surface proteins partially shared with tachyzoites. SAG1, SRS3 and SAG3 were detected on sporozoites as well as numerous minor antigens. In contrast, tachyzoite SAG2A and B were completely absent whereas a dominant 25\u2003kDa protein was unique to the sporozoite surface. The sporozoite gene encoding this protein was identified in tachyzoites genetically complemented with a sporozoite cDNA library and cloned via site\u2010specific recombination into a bacterial shuttle vector. The sporozoite cDNA identified in these experiments encoded a protein with conserved structural features of the prototypical <jats:italic>T. gondii<\/jats:italic> SAG1 (P30) and shared sequence identity with surface proteins from <jats:italic>Sarcocystis<\/jats:italic> spp. This new member of the SAG superfamily was designated SporoSAG. Expression of SporoSAG in tachyzoites conferred enhanced invasion on transgenic parasites suggesting a role for this protein in oocyst\/sporozoite transmission to susceptible hosts.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2003.03967.x","type":"journal-article","created":{"date-parts":[[2004,12,27]],"date-time":"2004-12-27T23:00:22Z","timestamp":1104188422000},"page":"93-105","source":"Crossref","is-referenced-by-count":61,"title":["Identification of a sporozoite\u2010specific member of the <i>Toxoplasma<\/i> SAG superfamily via genetic complementation"],"prefix":"10.1111","volume":"52","author":[{"given":"Jay R.","family":"Radke","sequence":"first","affiliation":[]},{"given":"Marc\u2010Jan","family":"Gubbels","sequence":"additional","affiliation":[]},{"given":"Maria E.","family":"Jerome","sequence":"additional","affiliation":[]},{"given":"Joshua 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