{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T16:06:57Z","timestamp":1764173217792},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,6,16]],"date-time":"2004-06-16T00:00:00Z","timestamp":1087344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>IS<jats:italic>231<\/jats:italic>A was originally discovered in <jats:italic>Bacillus thuringiensis<\/jats:italic> as a typical 1.6\u2003kb insertion sequence (IS) displaying 20\u2003bp inverted repeats (IR) flanking a transposase gene. A first major variation of this canonical organization was found in MIC<jats:italic>231<\/jats:italic>A1. This mobile insertion cassette (MIC), delineated by IS<jats:italic>231<\/jats:italic>A\u2010related extremities, contained an active <jats:sc>d<\/jats:sc>\u2010stereospecific endopeptidase (<jats:italic>adp<\/jats:italic>) gene instead of a transposase. Interestingly, it was shown that MIC<jats:italic>231<\/jats:italic>A1 can be mobilized <jats:italic>in trans<\/jats:italic> by the IS<jats:italic>231<\/jats:italic>A transposase. In this paper, we show that this family of IS<jats:italic>231<\/jats:italic>\u2013MIC<jats:italic>231<\/jats:italic> elements can be extended to a broad range of related entities displaying higher levels of structural complexity. Several IS<jats:italic>231<\/jats:italic>A\u2010like elements contained, upstream of their transposase gene, passenger genes coding for putative antibiotic resistances or regulatory factors. Furthermore, the diversity of the MIC<jats:italic>231<\/jats:italic> elements ranged from empty cassettes to structures carrying up to three passenger genes. Among these, MIC<jats:italic>231<\/jats:italic>V carried, in addition to an <jats:italic>adp<\/jats:italic> gene, an active fosfomycin resistance determinant. <jats:italic>In vivo<\/jats:italic> transposition assays showed that MIC<jats:italic>231<\/jats:italic>V is also <jats:italic>trans<\/jats:italic>\u2010activated by the IS<jats:italic>231<\/jats:italic>A transposase. These results lend further support to the potential contribution of these modular mobile elements to the genome plasticity of the <jats:italic>Bacillus cereus<\/jats:italic>\/<jats:italic>B. thuringiensis<\/jats:italic> group.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2004.04146.x","type":"journal-article","created":{"date-parts":[[2004,7,2]],"date-time":"2004-07-02T06:32:10Z","timestamp":1088749930000},"page":"457-467","source":"Crossref","is-referenced-by-count":34,"title":["IS<i>231<\/i>\u2013MIC<i>231<\/i> elements from <i>Bacillus cereus sensu lato<\/i> are modular"],"prefix":"10.1111","volume":"53","author":[{"given":"Daniel","family":"De 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