{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T05:35:11Z","timestamp":1776922511752,"version":"3.51.2"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Ultrarapid freezing of bacteria (i.e. vitrification) results in optimal preservation of native structure. In this study, cryo\u2010transmission electron microscopy of frozen\u2010hydrated sections was used to gain insight into the organization of the\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    168 cell envelope. A bipartite structure was seen above the plasma membrane consisting of a low\u2010density 22\u2003nm region above which a higher\u2010density 33\u2003nm region or outer wall zone (OWZ) resided. The interface between these two regions appeared to possess the most mass. In intact and in teichoic acid\u2010extracted wall fragments, only a single region was seen but the mass distribution varied from being dense on the inside to less dense on the outside (i.e. similar to the OWZ). In plasmolysed cells, the inner wall zone (IWZ)'s thickness expanded in size but the OWZ's thickness remained constant. As the IWZ expanded it became filled with plasma membrane vesicles indicating that the IWZ had little substance and was empty of the wall's polymeric network of peptidoglycan and teichoic acid. Together these results strongly suggest that the inner zone actually represents a periplasmic space confined between the plasma membrane and the wall matrix and that the OWZ is the peptidoglycan\u2010teichoic acid polymeric network of the wall.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2958.2005.04535.x","type":"journal-article","created":{"date-parts":[[2005,3,9]],"date-time":"2005-03-09T07:41:36Z","timestamp":1110354096000},"page":"240-251","source":"Crossref","is-referenced-by-count":262,"title":["Cryo\u2010electron microscopy reveals native polymeric cell wall structure in\n                    <i>Bacillus subtilis<\/i>\n                    168 and the existence of a periplasmic space"],"prefix":"10.1111","volume":"56","author":[{"given":"Val\u00e9rio R. 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