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Filamentation with polarization of actin patches was accompanied by the induction of the hypha\u2010specific genes (HSG) <jats:italic>ECE1<\/jats:italic>, <jats:italic>HWP1<\/jats:italic> and <jats:italic>HGC1<\/jats:italic>. However, filament formation occurred in the absence of the transcription factors Efg1 and Cph1, even though disruption of <jats:italic>EFG1<\/jats:italic> prevented expression of HSG. Therefore, expression of HSG genes accompanies but is dispensable for <jats:italic>rad52<\/jats:italic>\u0394 filamentation. However, deletion of adenylate cyclase severely impaired filamentation, this effect being largely reverted by the addition of exogenous cAMP. Filaments resembled elongated pseudohyphae, but some of them looked like true hyphae. Following depletion of Rad52, many cells arrested at the G2\/M phase of the cell cycle with a single nucleus suggesting the early induction of the DNA\u2010damage checkpoint. Filaments formed later, preferentially from G2\/M cells. The filamentation process was accompanied by the uncoupling of several landmark events of the cell cycle and was partially dependent on the action of the cell cycle modulator Swe1. Hyphae were still induced by serum, but a large number of <jats:italic>rad52<\/jats:italic> cells myceliated in G2\/M.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2005.05038.x","type":"journal-article","created":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T19:33:49Z","timestamp":1137785629000},"page":"1452-1472","source":"Crossref","is-referenced-by-count":65,"title":["Rad52 depletion in <i>Candida albicans<\/i> triggers both the DNA\u2010damage checkpoint and filamentation accompanied by but independent of expression of hypha\u2010specific 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