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Sbi is expressed on the cell surface but its C\u2010terminal domain lacks motifs associated with wall or membrane anchoring of proteins in Gram\u2010positive bacteria. Cell\u2010associated Sbi fractionates with the cytoplasmic membrane and is not solubilized during protoplast formation. <jats:italic>S.\u2003aureus<\/jats:italic> expressing Sbi truncates of the C\u2010terminal Y domain allowed identification of residues that are required for association of Sbi with the membrane. Recombinant Sbi bound to purified cytoplasmic membrane material <jats:italic>in vitro<\/jats:italic> and to purified lipoteichoic acid. This explains how Sbi partitions with the membrane in fractionation experiments yet is partially exposed on the cell surface. An LTA\u2010defective mutant of <jats:italic>S.\u2003aureus<\/jats:italic> had reduced levels of Sbi in the cytoplasmic membrane.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07966.x","type":"journal-article","created":{"date-parts":[[2012,1,19]],"date-time":"2012-01-19T05:32:03Z","timestamp":1326951123000},"page":"789-804","source":"Crossref","is-referenced-by-count":47,"title":["The immune evasion protein Sbi of <i>Staphylococcus aureus<\/i> occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid"],"prefix":"10.1111","volume":"83","author":[{"given":"Emma Jane","family":"Smith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca M.","family":"Corrigan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetje","family":"van der 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