{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:03:48Z","timestamp":1761743028288},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,10,9]],"date-time":"2007-10-09T00:00:00Z","timestamp":1191888000000},"content-version":"vor","delay-in-days":7405,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[1987,7]]},"abstract":"<jats:p><jats:bold>Summary<\/jats:bold> Of the possible iodine\u2010labelled <jats:italic>Toxoplasma gondii<\/jats:italic> surface proteins, P30 (apparent Mr 30000) is the principal one recognized by acute and convalescent anti\u2010toxoplasma sera. This protein which comprises from 3 to 5% of the total parasite protein was used to raise a panel of parasiticidal monoclonal anti\u2010P30 antibodies. One of these monoclonal antibodies was able to select a resistant mutant from a large population of chemically mutagenized wild\u2010type P strain parasites. This mutant retained the wild type sensitivity to other non\u2010P30 parasiticidal monoclonal antibodies as well as polyclonal anti\u2010P30 rabbit sera. Analysis of surface radioiodinated wild type and mutant parasites showed that the mutant had a quantitative reduction in the amount of P30. A comparison of surface biotin labelled wild type and resistant parasites by two dimensional electrophoresis showed that the mutant lacked one and possibly two of several proteins that make up wild type P30. Western blot analysis indicated that the mutant was devoid of antigenically reactive P30. These findings further support the hypothesis that antigenic variants of <jats:italic>T. gondii<\/jats:italic> can be induced and may involve the major surface membrane antigens of the parasite.<\/jats:p>","DOI":"10.1111\/j.1365-3024.1987.tb00521.x","type":"journal-article","created":{"date-parts":[[2007,10,9]],"date-time":"2007-10-09T14:14:22Z","timestamp":1191939262000},"page":"433-445","source":"Crossref","is-referenced-by-count":35,"title":["Isolation and characterization of a monoclonal anti\u2010P30 antibody resistant mutant of <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"9","author":[{"given":"LLOYD H.","family":"KASPER","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2007,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90320-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(85)90068-7"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/300019a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.164.3878.432"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(85)90287-X"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.4049\/jimmunol.129.4.1694","article-title":"Isolation and characterization of a monoclonal antibody resistant antigenic mutant of Toxoplasma gondii","volume":"129","author":"Kasper L. H.","year":"1982","journal-title":"Journal of Immunology"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.4049\/jimmunol.130.5.2407","article-title":"Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody","volume":"130","author":"Kasper L. H.","year":"1983","journal-title":"Journal of Immunology"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"3426","DOI":"10.4049\/jimmunol.134.5.3426","article-title":"An unexpected response to vaccination with a purified major membrane tachyzoite antigen (P30) of Toxoplasma gondii","volume":"134","author":"Kasper L. H.","year":"1985","journal-title":"Journal of Immunology"},{"key":"e_1_2_1_10_1","first-page":"346","article-title":"Proliferative T\u2010cell response to a purified membrane antigen (P30) of Toxoplasma gondii","volume":"33","author":"Kasper L. H.","year":"1985","journal-title":"Clinical Research"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(58)90805-5"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","article-title":"High resolution two\u2010dimensional electrophoresis","volume":"250","author":"O'Farrell P. H.","year":"1975","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470720806.ch5"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830150721"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1941.02820090001001"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1986.tb00876.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"977","DOI":"10.4049\/jimmunol.131.2.977","article-title":"Western blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies","volume":"131","author":"Sharma S. D.","year":"1983","journal-title":"Journal of Immunology"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198502213120806"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.4049\/jimmunol.101.1.166","article-title":"Comparative antigenic study of Bensnoitia jellisoni, B. panamenia, and five Toxoplasma gondii isolated","volume":"101","author":"Suggs M.","year":"1968","journal-title":"Journal of Immunology"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications","volume":"76","author":"Towbin M.","year":"1979","journal-title":"Proceedings of the National Acadamy of Science USA"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1128\/iai.55.3.778-783.1987","article-title":"Strain specific antigens of Toxoplasma gondii","volume":"55","author":"Ware P. L.","year":"1987","journal-title":"Infection and Immunity"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3024.1987.tb00521.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3024.1987.tb00521.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T10:09:34Z","timestamp":1697882974000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3024.1987.tb00521.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,7]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,7]]}},"alternative-id":["10.1111\/j.1365-3024.1987.tb00521.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3024.1987.tb00521.x","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,7]]}}}