{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:42:39Z","timestamp":1774676559905,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,6,29]],"date-time":"2006-06-29T00:00:00Z","timestamp":1151539200000},"content-version":"vor","delay-in-days":9585,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[1980,4]]},"abstract":"<jats:p>Human peripheral blood lymphocytes (PBL) from healthy donors express enhanced natural cytotoxicity to target cells after a brief exposure 10 mumps virus in vitro, We describe here experiments aiming at elucidating the mechanism of this virus\u2010dependant cytotoxicity. Treatment with proteolytic enzymes resulted in virus particles depleted of one or both kinds of their glycoprotein spikes. Removal of both of these components from the virion abrogated their ability to enhance cytotoxicity. This virus\u2010dependent cytotoxicity was significantly but not completed reduced when one of the spike glycoproteins (gp 75, HANA) was removed selectively. Similarly, nucleic\u2010acid\u2010free preparations of the spikes, obtained by detergent treatment of mumps virions, also dialed enhanced cytotoxicity. However, the activity of these preparations was lower than that of untreated virions Further evidence for the importance of II AN A was provided by the use of F(ab\u2032)<jats:sub>2<\/jats:sub> fragments of anti\u2010HANA\u2010specific rabbit antibodies. When these fragments were allowed to react with virus before addition of the virus to PBL. no augmentation of cytolysis was observed. Antibody fragments specific for the other spike protein (gp 61, F) failed to inhibit the virus\u2010dependent enhancement of PBL\u2010mediated cytotoxicity. However, anti\u2010HANA and anti\u2010F blocked this reaction when added directly to the mixture of virus\u2010treated PBL and target cells. The results are compatible with the hypothesis that virus\u2010dependent cytotoxicity requires HANA for anchoring the virus to PBL receptors (and perhaps to bring effector and target cells into closer contact), whereas F may be involved in subsequent events increasing effector cell function.<\/jats:p>","DOI":"10.1111\/j.1365-3083.1980.tb00005.x","type":"journal-article","created":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T14:09:15Z","timestamp":1151676555000},"page":"391-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["The Role of Viral Glycoproteins in Mumps\u2010Virus\u2010dependent Lymphocyte\u2010mediated Cytotoxicity in Vitro"],"prefix":"10.1111","volume":"11","author":[{"given":"B.","family":"H\u00c4RFAST","sequence":"first","affiliation":[]},{"given":"C.","family":"\u00d6RVELL","sequence":"additional","affiliation":[]},{"given":"A.","family":"ALSHEIKHLY","sequence":"additional","affiliation":[]},{"given":"T.","family":"ANDERSSON","sequence":"additional","affiliation":[]},{"given":"P.","family":"PERLMANN","sequence":"additional","affiliation":[]},{"given":"E.","family":"NORRBY","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,6,29]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.4049\/jimmunol.114.1_Part_1.237","article-title":"An in vitro method for study of human lymphocyte cytotoxicity against mumps virus infected target cells","volume":"114","author":"Andersson T.","year":"1975","journal-title":"J. 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