{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T17:10:21Z","timestamp":1699636221536},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,6,29]],"date-time":"2006-06-29T00:00:00Z","timestamp":1151539200000},"content-version":"vor","delay-in-days":9463,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[1980,8]]},"abstract":"<jats:p>In a recent report it was shown that what appeared to be secondary cytotoxic T cells could be obtained from lymphocytic choriomeningitis virus (LCMV)\u2010primed splenocytes after stimulation in vitro with the non\u2010specific T cell mitogen concanavalin A (Con A). The present experiments attempt to characterize further these effector cells and, in particular, to establish whether the Con A\u2010activated cytotoxic effectors are qualitatively different from the secondary cytotoxic T cells induced by restimulation with the homologous antigen. It was found that: (1) in vitro activation with Con A could be obtained with populations harvested between 13 days (the earliest tested) and at least 300 days after priming: (2) cytotoxicity was independent of the presence of carried\u2010over Con A in the cytotoxicity assay; (3) cytotoxicity was dependent on close association between activated T cells and target cells, since no evidence was found to indicate a role for other cell types or soluble (cytotoxic or arming) factors; (4) cytotoxicity was specific with regard to both virus and \u2018self\u2019. By comparison with previous data on LCMV\u2010induced cytotoxic T cells, it is concluded that Con A induces the generation of cytotoxic T cells from LCMV\u2010primed splenocytes, which, by the criteria used, are indistinguishable from virus\u2010induced secondary cytotoxic T cells. The implications of these findings are discussed.<\/jats:p>","DOI":"10.1111\/j.1365-3083.1980.tb00047.x","type":"journal-article","created":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T15:04:00Z","timestamp":1151679840000},"page":"109-118","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Concanavalin A\u2010mediated in Vitro Activation of a Secondary Cytotoxic T\u2010Cell Response in Virus\u2010primed Splenocytes"],"prefix":"10.1111","volume":"12","author":[{"given":"A. RANDRUP","family":"THOMSEN","sequence":"first","affiliation":[]},{"given":"B. 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