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In this study, the NK cell\u2010mediated killing of liver\u2010derived cells was investigated at oxygen concentrations conform to those present in the human body at physiological and pathological conditions. The <jats:italic>in vivo<\/jats:italic>\u2010relevant oxygen concentrations corresponding to 1, 2 and 6% were compared to those of the ambient air (21%) for their effect on the lysis of four liver\u2010derived cell lines and the highly susceptible K562 cells. Exposure to each of the hypoxic conditions had a significantly inhibitory effect on NK cytotoxicity. Treatment with interferon\u2010\u03b1 (IFN\u2010\u03b1) in hypoxia enhanced the cytotoxic potential of the NK cells less than it enhanced the cytotoxicity at ambient oxygen conditions. In summary, the oxygen tension profoundly affects both the cytoxic activity of NK cells and their activation by IFN\u2010\u03b1.<\/jats:p>","DOI":"10.1111\/j.1365-3083.2003.01347.x","type":"journal-article","created":{"date-parts":[[2003,11,26]],"date-time":"2003-11-26T01:19:16Z","timestamp":1069809556000},"page":"607-612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Natural Killer Cell\u2010Mediated Basal and Interferon\u2010Enhanced Cytotoxicity against Liver Cancer Cells is Significantly Impaired Under <i>In Vivo<\/i> Oxygen 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