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BrdU patterns in wild\u2010type plants were correlated with the expression profiles of both several cell cycle genes involved in the control of the G<jats:sub>1<\/jats:sub>\/S transition and cell cycle\u2010related repressor genes, <jats:italic>MSI4<\/jats:italic> and <jats:italic>MSI5<\/jats:italic>, encoding WD\u2010repeat proteins. To evaluate how proliferation patterns arise with respect to boundaries and vice versa, the expression of a boundary gene, <jats:italic>CUP SHAPED COTYLEDON<\/jats:italic> (<jats:italic>CUC<\/jats:italic>)<jats:italic>2<\/jats:italic>, was determined. Combining these approaches, we demonstrate that boundaries between inflorescence and floral meristems and between floral whorls are narrow bands of non\u2010dividing cells. In addition, we show that negative and positive regulators of cell proliferation are simultaneously and continuously expressed in dividing meristematic domains, being excluded from boundary cells. Finally, BrdU incorporation and <jats:italic>CUC2 in\u2003situ<\/jats:italic> hybridisation patterns were analysed in two mutant backgrounds, <jats:italic>agamous<\/jats:italic> (<jats:italic>ag<\/jats:italic>)<jats:italic>\u20101<\/jats:italic> and <jats:italic>superman<\/jats:italic> (<jats:italic>sup<\/jats:italic>)<jats:italic>\u20101<\/jats:italic>, in order to assess changes in boundary establishment and different levels of indeterminacy under conditions of altered proliferation at the floral meristem centre.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2004.02026.x","type":"journal-article","created":{"date-parts":[[2004,3,30]],"date-time":"2004-03-30T07:38:56Z","timestamp":1080632336000},"page":"182-192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":91,"title":["High\u2010resolution boundary analysis during <i>Arabidopsis thaliana<\/i> flower 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