{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T16:00:43Z","timestamp":1776268843362,"version":"3.50.1"},"reference-count":70,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,28]],"date-time":"2005-02-28T00:00:00Z","timestamp":1109548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Comparative genomics provides a tool to utilize the exponentially increasing sequence information from model plants to clone agronomically important genes from less studied crop species. Plant disease resistance (<jats:italic>R<\/jats:italic>) loci frequently lack synteny between related species of cereals and crucifers but appear to be positionally well conserved in the Solanaceae. In this report, we adopted a local RGA approach using genomic information from the model Solanaceous plant tomato to isolate <jats:italic>R3a<\/jats:italic>, a potato gene that confers race\u2010specific resistance to the late blight pathogen <jats:italic>Phytophthora infestans<\/jats:italic>. <jats:italic>R3a<\/jats:italic> is a member of the <jats:italic>R3<\/jats:italic> complex locus on chromosome 11. Comparative analyses of the <jats:italic>R3<\/jats:italic> complex locus with the corresponding <jats:italic>I2<\/jats:italic> complex locus in tomato suggest that this is an ancient locus involved in plant innate immunity against oomycete and fungal pathogens. However, the <jats:italic>R3<\/jats:italic> complex locus has evolved after divergence from tomato and the locus has experienced a significant expansion in potato without disruption of the flanking colinearity. This expansion has resulted in an increase in the number of <jats:italic>R<\/jats:italic> genes and in functional diversification, which has probably been driven by the co\u2010evolutionary history between <jats:italic>P. infestans<\/jats:italic> and its host potato. Constitutive expression was observed for the <jats:italic>R3a<\/jats:italic> gene, as well as some of its paralogues whose functions remain unknown.<\/jats:p>","DOI":"10.1111\/j.1365-313x.2005.02365.x","type":"journal-article","created":{"date-parts":[[2005,3,30]],"date-time":"2005-03-30T15:51:50Z","timestamp":1112197910000},"page":"251-261","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":315,"title":["Comparative genomics enabled the isolation of the <i>R3a<\/i> late blight resistance gene in potato"],"prefix":"10.1111","volume":"42","author":[{"given":"Sanwen","family":"Huang","sequence":"first","affiliation":[]},{"given":"Edwin A.G.","family":"Van Der Vossen","sequence":"additional","affiliation":[]},{"given":"Hanhui","family":"Kuang","sequence":"additional","affiliation":[]},{"given":"Vivianne G.A.A.","family":"Vleeshouwers","sequence":"additional","affiliation":[]},{"given":"Ningwen","family":"Zhang","sequence":"additional","affiliation":[]},{"given":"Theo J.A.","family":"Borm","sequence":"additional","affiliation":[]},{"given":"Herman J.","family":"Van Eck","sequence":"additional","affiliation":[]},{"given":"Barbara","family":"Baker","sequence":"additional","affiliation":[]},{"given":"Evert","family":"Jacobsen","sequence":"additional","affiliation":[]},{"given":"Richard G.F.","family":"Visser","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,3,7]]},"reference":[{"key":"e_1_2_19_2_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1998.11.4.251"},{"key":"e_1_2_19_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_19_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35048692"},{"key":"e_1_2_19_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.016725"},{"key":"e_1_2_19_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2001.01292.x"},{"key":"e_1_2_19_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-4-489"},{"key":"e_1_2_19_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061337"},{"key":"e_1_2_19_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/120.4.1095"},{"key":"e_1_2_19_10_1","doi-asserted-by":"publisher","DOI":"10.1139\/g04-001"},{"key":"e_1_2_19_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.0951"},{"key":"e_1_2_19_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2218528"},{"key":"e_1_2_19_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/35081161"},{"key":"e_1_2_19_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.1.13"},{"key":"e_1_2_19_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00283432"},{"key":"e_1_2_19_16_1","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1007\/BF00221901","article-title":"R6 and R7 alleles of potato conferring race\u2010specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI","volume":"92","author":"El Kharbotly A.","year":"1996","journal-title":"Theor. 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