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Purified histones were added during the extraction regime to assess endogenous proteolytic activity and to serve as internal markers. Removal of proteins by 0.4 M NaCl from either chromatin resulted in little or no change in original template activity, solubility and thermal denaturation properties. Significant changes in these parameters were observed after extraction with NaCl  0.6 M. For liver chromatin, template activity and thermal melting properties gradually approached those of deproteinized DNA. The largest change occurred after the extraction of non\u2010histone proteins which are not removed during 2.0\u2010M NaCl treatment. In contrast, the largest single change in erythrocyte template properties occurred with 0.6\u2010M NaCl treatment. At this salt concentration, erythrocyte template availability was the same as for liver chromatin. The only major component extracted was the erythrocyte specific histone, V or f2c. Annealing studies using saturating amounts of labelled RNA synthesized from the various types of chromatin confirmed the template activity studies. More of the DNA in chromatin appears to be transcribed as chromatin is extracted with increasingly higher concentrations of salt, with little indication that reassociation of protein and loss of transcripts occurs. Pre\u2010saturation competition studies using [<jats:sup>3<\/jats:sup>H]RNA synthesized from 0.6\u2010M and 2.0\u2010M salt\u2010extracted chromatin indicates that histones are probably more important for maintaining the highly restricted template of the mature erythrocyte chromatin than for the more active template of liver chromatin.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1974.tb03618.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T13:39:24Z","timestamp":1109943564000},"page":"259-269","source":"Crossref","is-referenced-by-count":14,"title":["Comparison of Template\u2010Property Changes after Salt Extraction of Avian Erythrocyte and Liver Chromatin"],"prefix":"10.1111","volume":"46","author":[{"given":"Verner L.","family":"SELIGY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"MIYAGI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(66)90140-9"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(67)12116-2"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00832a043"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1969.tb00661.x"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1200859"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(71)90184-X"},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90421-7"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(69)90338-6"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1070207"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(70)90046-8"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1972.tb02010.x"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/206680a0"},{"key":"e_1_2_3_14_2","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1432-1033.1971.tb01537.x","volume":"22","author":"Ilyia Y. 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