{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:21:49Z","timestamp":1771640509615,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":11136,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1978,1]]},"abstract":"<jats:p>We have compared the mitogenic activity and reactivity in receptor binding assays of somatomedin A and multiplication\u2010stimulating activity (MSA), two acid\u2010soluble polypeptides of molecular weight 7\u201310 000 that possess weak intrinsic insulin\u2010like metabolic activity. Somatomedin A was purified to homogeneity from human plasma, and MSA was purified to homogeneity from the conditioned culture media of a cell line, BRL 3A, derived from rat liver. The two peptides stimulated DNA synthesis in chick embryo fibroblasts 3\u20134\u2010fold and have superimposable dose\u2010response curves. Addition of either peptide to chick embryo fibroblasts plated in serum\u2010free medium increased the cell number by 150% in 4 days, compared with an increase of 25% in control cultures receiving no additions. The two peptides also stimulated DNA synthesis in human fibroblasts in culture 3\u20134\u2010fold.<\/jats:p><jats:p>Specific receptors for MSA and\/or somatomedin A could be demonstrated in intact cells or membranes from chick embryo fibroblasts, human fibroblasts, human placenta, rat liver, and the BRL 3A2 cell line, a subclone of the line that produces MSA. Unlabeled MSA and somatomedin A inhibited the binding of <jats:sup>125<\/jats:sup>I\u2010labeled MSA and <jats:sup>125<\/jats:sup>I\u2010labeled somatomedin A to each of these receptors with comparable potency. In chick embryo fibroblasts, human fibroblasts, and human placental membranes, the binding of both radioactive ligands also was inhibited by insulin, consistent with the interpretation that <jats:sup>125<\/jats:sup>I\u2010labeled MSA and <jats:sup>125<\/jats:sup>I\u2010labeled somatomedin A were binding to the same receptor. By contrast, in the BRL 3A2 cell line, insulin inhibited the binding of <jats:sup>125<\/jats:sup>I\u2010labeled somatomedin A, but not the binding of <jats:sup>125<\/jats:sup>I\u2010labeled MSA, suggesting that the two labeled peptides were binding to different receptors in this cell line. Moreover, <jats:sup>125<\/jats:sup>I\u2010labeled MSA, but not <jats:sup>125<\/jats:sup>I\u2010labeled somatomedin A, bound specifically to rat liver plasma membranes. These results indicate that human somatomedin A and rat MSA are closely related, but not identical, peptides. They have similar mitogenic activity for cultured fibroblasts, and cross\u2010react with specific cell receptors with comparable potency. The two radioactively labeled peptides, however, can be distinguished by certain cell surface receptors for somatomedin\u2010like peptides.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1978.tb11991.x","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T18:21:45Z","timestamp":1109874105000},"page":"5-12","source":"Crossref","is-referenced-by-count":58,"title":["Purified Human Somatomedin A and Rat Multiplication Stimulating Activity"],"prefix":"10.1111","volume":"82","author":[{"given":"Matthew M.","family":"RECHLER","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"FRYKLUND","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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