{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T21:30:28Z","timestamp":1767648628261},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":10712,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1979,3]]},"abstract":"<jats:p>Mitochondrial mutants of <jats:italic>Saccharomyces cerevisiae<\/jats:italic> defective in cytochrome <jats:italic>b<\/jats:italic> were analyzed genetically and biochemically in order to elucidate the role of the mitochondrial genetic system in the biosynthesis of this cytochrome.<\/jats:p><jats:p>The mutants mapped between <jats:italic>OLI1<\/jats:italic> and <jats:italic>OLI2<\/jats:italic> on mitochondrial DNA in a region called <jats:italic>COB<\/jats:italic>. A fine structure map of the <jats:italic>COB<\/jats:italic> region was constructed by <jats:italic>rho<\/jats:italic><jats:sup>\u2212<\/jats:sup> deletion mapping and recombination analysis.<\/jats:p><jats:p>The combined genetic and biochemical data indicate that the <jats:italic>COB<\/jats:italic> region is mosaic and contains at least five distinct clusters of mutants, A\u2013E, with A being closest to <jats:italic>OLI2<\/jats:italic> and E being closest to <jats:italic>OLI1<\/jats:italic>. Clusters A, C and E are probably coding regions for apocytochrome <jats:italic>b<\/jats:italic>, whereas clusters B and D seem to be involved in as yet unknown functions. These conclusions rest on the following evidence.<\/jats:p><jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Most mutants in clusters A, C and E have specifically lost cytochrome <jats:italic>b<\/jats:italic>. Many of them accumulate smaller mitochondrial translation products; some of these were identified as fragments of apocytochrome <jats:italic>b<\/jats:italic> by proteolytic fingerprinting. The molecular weight of these fragments depends on the map position of the mutant, increasing in the direction <jats:italic>OLI2\u2192OLI1<\/jats:italic>. The mutant closest to <jats:italic>OLI1<\/jats:italic> accumulates an apocytochrome <jats:italic>b<\/jats:italic> which is slightly larger than that of wild type.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A mutant in cluster C exihibits a spectral absorption band of cytochrome <jats:italic>b<\/jats:italic> that is shifted 1.5 nm to the red.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Mutants in clusters B and D are pleiotropic. A majority of them are conditional and lack the absorption bands of both cytochrome <jats:italic>b<\/jats:italic> and cytochrome <jats:italic>aa<\/jats:italic><jats:sub>3<\/jats:sub>; these mutants also fail to accumulate apocytochrome <jats:italic>b<\/jats:italic> and subunit I of cytochrome <jats:italic>c<\/jats:italic> oxidase and instead form a large number of abnormal translation products whose nature is unknown.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Zygotic complementation tests reveal at leas two complementation groups: The first group includes all mutants in cluster B and the second group includes mutants in clusters (A+C+D+E).<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1432-1033.1979.tb12913.x","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T19:55:37Z","timestamp":1109879737000},"page":"451-464","source":"Crossref","is-referenced-by-count":91,"title":["The Mitochondrial <i>COB<\/i> Region in Yeast Codes for Apocytochrome <i>b<\/i> and Is Mosaic"],"prefix":"10.1111","volume":"94","author":[{"given":"Albert","family":"HAID","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf J.","family":"SCHWEYEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helga","family":"BECHMANN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fritz","family":"KAUDEWITZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"SOLIOZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gottfried","family":"SCHATZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.43.070174.000411"},{"key":"e_1_2_2_3_2","volume-title":"The Genetic Function of Mitochondrial DNA","author":"Saccone C.","year":"1976"},{"key":"e_1_2_2_4_2","volume-title":"Genetics and Biogenesis of Chloroplasts and Mitochondria","author":"B\u00fccher Th.","year":"1976"},{"key":"e_1_2_2_5_2","doi-asserted-by":"crossref","DOI":"10.1515\/9783111533391","volume-title":"Mitochondria 1977","author":"Bandlow W.","year":"1977"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03244.x"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1976.tb11031.x"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1976.tb09994.x"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00275958"},{"key":"e_1_2_2_10_2","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1515\/9783111533391-026","volume-title":"Mitochondria 1977","author":"Claisse M.","year":"1977"},{"key":"e_1_2_2_11_2","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1515\/9783111533391-027","volume-title":"Mitochondria 1977","author":"Mahler H. 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