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Using isotonic ammonium chloride treatment which renders the plasma membrane of thymocytes permeable to sugar nucleotides, we demonstrate that, in contrast to GDP\u2010Man, a pool of UDP\u2010GlcNAc remains associated with the cells after plasma membrane permeabilization. These observations are confirmed in experiments in which permeabilized thymocytes are incubated with exogenous labelled GDP\u2010Man and UDP\u2010GlcNAc, and we show that only UDP\u2010GlcNAc is accumulated into sealed intracellular vesicles. This accumulation is a saturable process which can be inhibited by UDP, demonstrating the occurrence of a specific carrier. This transport mechanism can be blocked by covalent attachment of a non\u2010permeant inhibitor UDP\u2010dialdehyde without affecting the <jats:italic>N<\/jats:italic>\u2010acetylglucosaminyltransferase itself. The fact that this carrier\u2010mediated transport is not inhibited by tunicamycin indicates that this translocation process of UDP\u2010GlcNAc does not involve lipid intermediates.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1985.tb09275.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T00:13:36Z","timestamp":1109895216000},"page":"111-116","source":"Crossref","is-referenced-by-count":9,"title":["Accumulation of UDP\u2010GlcNAc into intracellular vesicles and occurrence of a carrier\u2010mediated transport"],"prefix":"10.1111","volume":"153","author":[{"given":"Rom\u00e9o","family":"CECCHELLI","sequence":"first","affiliation":[]},{"given":"Ren\u00e9","family":"CACAN","sequence":"additional","affiliation":[]},{"given":"Andr\u00e9","family":"VERBERT","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)33897-3"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.22.7051"},{"key":"e_1_2_2_4_2","first-page":"163","volume-title":"Falk symposium on structural carbohydrates in the liver","author":"Fleischer B.","year":"1983"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1680423"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(81)90423-9"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90355-6"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)81032-3"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2160681"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2240277"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1983.tb07373.x"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1980.tb04594.x"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1979.sp012747"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(75)90190-6"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00507a050"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85579-5"},{"key":"e_1_2_2_17_2","doi-asserted-by":"crossref","first-page":"3926","DOI":"10.1016\/S0021-9258(17)40339-5","volume":"252","author":"Narasimhan S.","year":"1977","journal-title":"J. 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