{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:03:31Z","timestamp":1758279811252},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T00:00:00Z","timestamp":1109808000000},"content-version":"vor","delay-in-days":6667,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1986,12]]},"abstract":"<jats:p>The properties of EF\u20101\u03b1 from calf brain have been investigated and compared with those of EF\u2010Tu. EF\u20101\u03b1 binds GDP and GTP in a 1:1 stoichiometry, showing the same affinity for both nucleotides (<jats:italic>K\u2032<\/jats:italic><jats:sub>d<\/jats:sub>= 2\u20134 \u03bcM). EF\u20101\u03b2 strongly enhances the dissociation rate of the EF\u20101\u03b1\u00b7 GDP complex and to a lesser extent of the EF\u20101\u03b1\u00b7 GTP complex. Aminoacyl\u2010tRNA (aa\u2010tRNA) stabilizes EF\u20101\u03b1\u00b7 GTP much less efficiently than the EF\u2010Tu \u00b7 GTP complex. Unlike EF\u2010Tu, EF\u20101\u03b1 sustains the binding of aa\u2010tRNA to the ribosome also in the presence of GDP or in the absence of any nucleotide, though to a lesser degree than with GTP. Kirromycin enhances the dissociation rate of both EF\u20101\u03b1\u00b7 GTP and EF\u20101\u03b1\u00b7 GDP but especially that of the latter. This effect results in an increase of the exchange rate of the EF\u20101\u03b1\u2010bound nucleotide with free nucleotides. Although in this regard the effect of kirromycin mimicks that of EF\u20101\u03b2, the antibiotic is incapable of increasing the EF\u20101\u03b1\u00b7 GDP\/GTP exchange rate when aa\u2010tRNA and ribosomes are present. Therefore, unlike EF\u20101\u03b2, kirromycin cannot enhance the rate of poly(Phe) synthesis. On the other hand, the failure of kirromycin to induce a GTP\u2010like conformation of EF\u20101\u03b1\u00b7 GDP, as in the case of EF\u2010Tu \u00b7 GDP, explains its inability to inhibit peptide bond formation in the eukaryotic system.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1986.tb10489.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T00:59:54Z","timestamp":1109897994000},"page":"647-653","source":"Crossref","is-referenced-by-count":29,"title":["Characterization of the elongation factors from calf brain"],"prefix":"10.1111","volume":"161","author":[{"given":"Jean\u2010Bernard","family":"CRECHET","sequence":"first","affiliation":[]},{"given":"Andrea","family":"PARMEGGIANI","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/258452a0"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1984.tb07967.x"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(75)90336-7"},{"key":"e_1_2_2_5_2","first-page":"1979","volume":"38","author":"Moldave K.","year":"1979","journal-title":"Fed. 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Functional properties of EF\u20101\u03b1, the action of physiological ligands and kirromycin"],"short-title":[],"issued":{"date-parts":[[1986,12]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1986,12]]}},"alternative-id":["10.1111\/j.1432-1033.1986.tb10489.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1432-1033.1986.tb10489.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,12]]}}}