{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T03:40:29Z","timestamp":1770694829824,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T00:00:00Z","timestamp":1109808000000},"content-version":"vor","delay-in-days":6058,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1988,8]]},"abstract":"<jats:p>Exposure of Chinese hamster ovary, mouse adrenal cortex tumor (Y\u20101), THP\u20101 and U\u2010937 cells and human erythrocytes to adenylate\u2010cyclase\u2010containing urea extracts of <jats:italic>Bordetella pertussis<\/jats:italic> (strain 114) organisms promotes the formation of large concentrations of intracellular CAMP. Accumulation is dependent on dose and temperature, with significant accumulation occurring at 4\u00b0 C, and is virtually instantaneous, with a doubling at 1 min. There is an absolute Ca<jats:sup>2+<\/jats:sup> requirement but external calmodulin (the activator of cyclase activity) has no effect except in erythrocytes and U\u2010937 cells, where it reduces <jats:sc>C<\/jats:sc>AMP accumulation. However, calmodulin antagonists inhibit cAMP accumulation. In Y\u20101 adrenal cells the urea\u2010extract adenylate cyclase stimulates steroidogenesis.<\/jats:p><jats:p>Anti\u2010(<jats:italic>B. pertussis<\/jats:italic>) antibodies inhibit cyclase activity and prevent further cAMP accumulation after 10 min in cells previously exposed to urea extract. The same effect is obtained by washing. This suggests that a portion of the cyclase is associated with cells in a form not accessible to antibody or washing but accessible to substrate, which we interpret as internalized enzyme with a short lifetime. Continuing <jats:sc>C<\/jats:sc>AMP accumulation thus appears to need a continuing source of external cyclase.<\/jats:p><jats:p>Inhibitors of the effect of diphtheria toxin, such as NH<jats:sub>4<\/jats:sub>Cl, methylamine, chloroquin or monensin, have no inhibitory effect on the accumulation of intracelluar cAMP promoted by the internalized adenylate cyclase of urea extracts of <jats:italic>B. pertussis<\/jats:italic> organisms. We conclude that entry of the cyclase into cells is not by receptor\u2010mediated endocytosis.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1988.tb14215.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T02:21:29Z","timestamp":1109902889000},"page":"447-453","source":"Crossref","is-referenced-by-count":27,"title":["<i>Bordetella pertussis<\/i> adenylate cyclase"],"prefix":"10.1111","volume":"175","author":[{"given":"Fabrizio","family":"GENTILE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastassios","family":"RAPTIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie G.","family":"KNIPLING","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"WOLFF","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.6.1926"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6287574"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.7.3841"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/150.2.219"},{"key":"e_1_2_2_6_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1128\/iai.43.1.263-269.1984","volume":"43","author":"Weiss A. 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